市售细胞角蛋白抗体在正常和肿瘤性成人上皮及非上皮组织中的比较。
Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues.
作者信息
Goddard M J, Wilson B, Grant J W
机构信息
Department of Histopathology, Addenbrooke's Hospital, Cambridge.
出版信息
J Clin Pathol. 1991 Aug;44(8):660-3. doi: 10.1136/jcp.44.8.660.
Abstract
Five commercially available cytokeratin antibodies (lu-5, AE1/AE3, CAM 5.2, MFN116 and anti-cytokeratin 18) were used to stain a wide range of normal and neoplastic epithelial and non-epithelial tissues to assess their potential value in diagnostic histopathology. All five showed good specificity, with some cross-reactivity in smooth muscle cells. The wider reactivity of AE1/AE3, lu-5, and MFN 116, which includes cytokeratins 8,18 (Moll's catalogue) expressed in simple epithelia and their tumours, as well as cytokeratins expressed in complex stratified squamous epithelia, permits identification of a wider range of epithelial derived tumours. This wider spectrum of reactivity may allow these antibodies to be used in a diagnostic panel for the identification of poorly differentiated tumours.
摘要
使用五种市售的细胞角蛋白抗体（lu-5、AE1/AE3、CAM 5.2、MFN116和抗细胞角蛋白18）对多种正常和肿瘤性上皮及非上皮组织进行染色，以评估它们在诊断组织病理学中的潜在价值。所有五种抗体均表现出良好的特异性，在平滑肌细胞中有一些交叉反应性。AE1/AE3、lu-5和MFN 116具有更广泛的反应性，包括在单层上皮及其肿瘤中表达的细胞角蛋白8、18（莫尔分类），以及在复层鳞状上皮中表达的细胞角蛋白，这使得能够识别更广泛的上皮源性肿瘤。这种更广泛的反应谱可能使这些抗体可用于诊断组合，以识别低分化肿瘤。
相似文献
1
Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues.
J Clin Pathol. 1991 Aug;44(8):660-3. doi: 10.1136/jcp.44.8.660.
2
A comparison of six monoclonal antibodies for detection of cytokeratins in normal and neoplastic canine tissues.
J Vet Diagn Invest. 1989 Oct;1(4):316-23. doi: 10.1177/104063878900100407.
3
Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13.
Exp Cell Res. 1986 Jan;162(1):97-113. doi: 10.1016/0014-4827(86)90429-5.
4
Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.
Eur J Cancer Clin Oncol. 1987 Feb;23(2):137-47. doi: 10.1016/0277-5379(87)90007-1.
5
Rat antikeratin monoclonal antibodies are specific for stratified squamous epithelia.
Am J Clin Pathol. 1988 Mar;89(3):315-20. doi: 10.1093/ajcp/89.3.315.
6
Expression of simple epithelial type cytokeratins in stratified epithelia as detected by immunolocalization and hybridization in situ.
J Cell Biol. 1988 May;106(5):1635-48. doi: 10.1083/jcb.106.5.1635.
7
Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2.
Am J Clin Pathol. 1987 Sep;88(3):297-301. doi: 10.1093/ajcp/88.3.297.
8
A keratin epitope that is exposed in a subpopulation of preneoplastic and neoplastic mouse mammary epithelial cells but not in normal cells.
Cancer Res. 1986 Mar;46(3):1255-62.
9
Immunocytochemical study of tissues from clinically normal dogs and of neoplasms, using keratin monoclonal antibodies.
Am J Vet Res. 1991 Apr;52(4):613-8.
10
Monoclonal antibodies with different specificities against cytokeratins. An immunohistochemical study of normal tissues and tumors.
Am J Pathol. 1984 Jan;114(1):9-17.
引用本文的文献
1
Detection of Circulating Tumor Cell-Related Markers in Gynecologic Cancer Using Microfluidic Devices: A Pilot Study.
Int J Mol Sci. 2023 Jan 24;24(3):2300. doi: 10.3390/ijms24032300.
2
Proteomic analysis of tumor tissue in CT-26 implanted BALB/C mouse after treatment with ascorbic acid.
Cell Mol Biol Lett. 2012 Mar;17(1):62-76. doi: 10.2478/s11658-011-0035-7. Epub 2011 Dec 2.
3
A rationally designed histone deacetylase inhibitor with distinct antitumor activity against ovarian cancer.
Neoplasia. 2009 Jun;11(6):552-63, 3 p following 563. doi: 10.1593/neo.09204.
4
Molecular and pathological signatures of epithelial-mesenchymal transitions at the cancer invasion front.
Histochem Cell Biol. 2008 Sep;130(3):481-94. doi: 10.1007/s00418-008-0464-1. Epub 2008 Jul 22.
5
Breast cancer with an unusual leukemia-like presentation: case report and literature review.
Med Oncol. 2008;25(1):100-3. doi: 10.1007/s12032-007-0048-2. Epub 2007 Jul 28.
6
Immunohistochemical study of cytoskeletal and extracellular matrix components in the notochord and notochordal sheath of amphioxus.
Int J Biol Sci. 2006;2(2):73-8. doi: 10.7150/ijbs.2.73. Epub 2006 Apr 10.
7
[Optimization of staging in colon cancer using sentinel lymph node biopsy].
Chirurg. 2005 Jan;76(1):58-67. doi: 10.1007/s00104-004-0820-1.
8
A genetically retargeted adenoviral vector enhances viral transduction in esophageal carcinoma cell lines and primary cultured esophageal resection specimens.
Ann Surg. 2003 Dec;238(6):815-24; discussion 825-6. doi: 10.1097/01.sla.0000098622.47909.c0.
9
Capacity for epithelial differentiation in synovial sarcoma: analysis of a new human cell line.
J Clin Pathol. 2000 Jul;53(7):525-31. doi: 10.1136/jcp.53.7.525.
10
Cytokeratin demonstration in thick breast tissue slices.
Histochem J. 1997 May;29(5):409-12. doi: 10.1023/a:1026495002835.
本文引用的文献
1
Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.
Int J Cancer. 1981 Jul 15;28(1):17-21. doi: 10.1002/ijc.2910280104.
2
The differential diagnosis of routinely processed anaplastic tumors using monoclonal antibodies.
Am J Clin Pathol. 1984 Jul;82(1):33-43. doi: 10.1093/ajcp/82.1.33.
3
Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers.
Int J Cancer. 1981 Jul 15;28(1):23-9. doi: 10.1002/ijc.2910280105.
4
Monoclonal antibody to cytokeratin for use in routine histopathology.
J Clin Pathol. 1984 Sep;37(9):975-83. doi: 10.1136/jcp.37.9.975.
5
Identification of large cell undifferentiated tumours in lymph nodes using leucocyte common and keratin antibodies.
Histopathology. 1984 Mar;8(2):259-72. doi: 10.1111/j.1365-2559.1984.tb02340.x.
6
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
Cell. 1982 Nov;31(1):11-24. doi: 10.1016/0092-8674(82)90400-7.
7
Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.
J Clin Pathol. 1982 Nov;35(11):1253-67. doi: 10.1136/jcp.35.11.1253.
8
Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
J Cell Biol. 1982 Nov;95(2 Pt 1):580-8. doi: 10.1083/jcb.95.2.580.
9
Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.
Cell. 1982 Sep;30(2):361-72. doi: 10.1016/0092-8674(82)90234-3.
10
Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology.
Cancer. 1981 Apr 1;47(7):1786-95. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8.
Zotero 插件