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利用单倍体阶段表达的cDNA探针分析小鼠中的雄性不育突变。

Analysis of male sterile mutations in the mouse using haploid stage expressed cDNA probes.

作者信息

Dudley K, Potter J, Lyon M F, Willison K R

出版信息

Nucleic Acids Res. 1984 May 25;12(10):4281-93. doi: 10.1093/nar/12.10.4281.

DOI:10.1093/nar/12.10.4281
PMID:6203099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318831/
Abstract

A differential hybridization screening procedure has identified cDNAs which correspond to RNAs which are expressed in mouse testis and at lower levels in liver and spleen. The sensitivity of this procedure is such that approximately 0.5% of 1.4 X 10(4) cDNA clones are revealed as "testis specific". We have focused on ten cDNA clones which have been used to identify RNAs expressed in the haploid phase of spermatogenesis. Using Northern blots to analyse RNA isolated from the testes of mutant mice (Tfm/Y and Sxr/+) blocked at specific stages in spermatogenesis or RNA from sexually immature mice, 8 clones have been identified which correspond to RNAs expressed uniquely or at much higher levels in meiotic or post meiotic cells.

摘要

一种差异杂交筛选程序已鉴定出与在小鼠睾丸中表达且在肝脏和脾脏中表达水平较低的RNA相对应的cDNA。该程序的灵敏度使得在1.4×10⁴个cDNA克隆中约0.5%被揭示为“睾丸特异性”。我们聚焦于十个cDNA克隆，它们已被用于鉴定在精子发生单倍体阶段表达的RNA。使用Northern印迹法分析从在精子发生特定阶段受阻的突变小鼠（Tfm/Y和Sxr/+）的睾丸中分离的RNA或来自性未成熟小鼠的RNA，已鉴定出8个克隆，它们对应于在减数分裂或减数分裂后细胞中独特表达或表达水平高得多的RNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/2fbcead1af2b/nar00328-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/899fbd2a8b38/nar00328-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/4cf0a351a8d6/nar00328-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/3a9ccdeb8eec/nar00328-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/2fbcead1af2b/nar00328-0242-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/899fbd2a8b38/nar00328-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/4cf0a351a8d6/nar00328-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/3a9ccdeb8eec/nar00328-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f2e4/318831/2fbcead1af2b/nar00328-0242-a.jpg

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本文引用的文献

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The human homologue of the mouse t-complex gene, TCP1, is located on chromosome 6 but is not near the HLA region.小鼠t-复合体基因TCP1的人类同源基因位于6号染色体上，但不在HLA区域附近。
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