小鼠中对同基因睾丸细胞迟发型超敏反应的局部被动转移。
Local passive transfer of delayed type hypersensitivity to syngeneic testicular cells in mice.
作者信息
Sakamoto Y, Sanui H, Yoshida S, Nomoto K
出版信息
Clin Exp Immunol. 1984 May;56(2):353-60.
A means for local passive transfer (LPT) of delayed footpad reaction (DFR) against syngeneic testicular cells (TC) was established in mice. Peritoneal exudate (PE) cells from immunized donors pre-treated with cyclophosphamide (Cy) were transferred with syngeneic TC into a hind footpad of a naive mouse. Antigen specific DFR was successfully transferred by PE cells as early as 3-5 days after immunization and as late as 39 days after immunization. DFR was abolished after treatment of PE cells with anti-0 serum, indicating T cell dependence. No footpad reaction can be elicited in male mice immunized with syngeneic TC without Cy pre-treatment. However, PE cells of those mice can passively transfer footpad reaction locally, suggesting the existence of suppressive mechanism in the donors. Although no suppressive mechanism was disclosed at the present, this LPT system seems a potent tool for further analysis of the regulatory mechanisms.
在小鼠中建立了一种针对同基因睾丸细胞（TC）的迟发型足垫反应（DFR）的局部被动转移（LPT）方法。将经环磷酰胺（Cy）预处理的免疫供体的腹腔渗出液（PE）细胞与同基因TC一起转移到未免疫小鼠的后足垫中。早在免疫后3 - 5天以及晚至免疫后39天，PE细胞就成功转移了抗原特异性DFR。用抗θ血清处理PE细胞后，DFR消失，表明其依赖T细胞。未经Cy预处理而用同基因TC免疫的雄性小鼠不会引发足垫反应。然而，这些小鼠的PE细胞可以局部被动转移足垫反应，这表明供体中存在抑制机制。尽管目前尚未揭示抑制机制，但这种LPT系统似乎是进一步分析调节机制的有力工具。
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