Berger A E, Davis J E, Cresswell P
Hybridoma. 1982;1(2):87-90. doi: 10.1089/hyb.1.1982.1.87.
The monoclonal antibody GAP A3 detects the HLA allospecificity A3. Reactivity of the monoclonal was in exact concordance with the presence of the A3 antigen as defined by conventional alloantisera on a panel of 59 cells from individuals with well-characterized HLA antigens. Reactivity with GAP A3 segregated with HLA-A3 in a family where three of eight siblings inherited the paternal A3 antigen. GAP A3 precipitated appropriate 44,000- and 12,000-dalton bands on SDS-polyacrylamide gels under reducing conditions from an HLA-A3-positive, but not an HLA-A3-negative B lymphoblastoid cell line. Thus, by serological, familial, and biochemical criteria, GAP A3 defines the allospecificity HLA-A3.
单克隆抗体GAP A3可检测HLA同种特异性A3。在一组来自HLA抗原特征明确的个体的59个细胞上，该单克隆抗体的反应性与传统同种抗血清所定义的A3抗原的存在完全一致。在一个八名兄弟姐妹中有三人继承了父系A3抗原的家族中，与GAP A3的反应性与HLA - A3呈分离状态。在还原条件下，GAP A3在SDS - 聚丙烯酰胺凝胶上从HLA - A3阳性而非HLA - A3阴性的B淋巴母细胞系中沉淀出合适的44000道尔顿和12000道尔顿的条带。因此，根据血清学、家族性和生化标准，GAP A3定义了同种特异性HLA - A3。
