脊髓灰质炎病毒衣壳蛋白VP2上新的中和抗原位点的鉴定。
Identification of a new neutralization antigenic site on poliovirus coat protein VP2.
作者信息
Emini E A, Jameson B A, Wimmer E
出版信息
J Virol. 1984 Nov;52(2):719-21. doi: 10.1128/JVI.52.2.719-721.1984.
Abstract
Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and synthesis of a peptide (n = 12) that, after linking to a carrier protein, induced an antiviral neutralizing antibody response in rabbits. The response was augmented by a single subsequent inoculation of intact virus; thus, the peptide was also capable of priming the production of neutralizing antibodies. These antibodies were directed only against the site specified by the synthetic peptide. Although the VP2-specific neutralization antigenic site appears not to be strongly immunogenic in the intact virion, it can nevertheless contribute to neutralization of poliovirus. This observation may be important for the development of peptide vaccines.
摘要
我们之前已在脊髓灰质炎1型病毒最大的衣壳蛋白VP1上绘制出主要中和抗原位点。在此，我们报告首次鉴定出衣壳蛋白VP2上中和抗原位点的一级序列。对VP2氨基酸序列的检查促使我们选择并合成了一种肽（n = 12），该肽与载体蛋白连接后，在兔体内诱导出抗病毒中和抗体反应。通过随后单次接种完整病毒，反应得到增强；因此，该肽也能够引发中和抗体的产生。这些抗体仅针对合成肽所指定的位点。尽管VP2特异性中和抗原位点在完整病毒体中似乎免疫原性不强，但它仍可有助于脊髓灰质炎病毒的中和。这一观察结果可能对肽疫苗的开发具有重要意义。
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