平原毛丝鼠、猫、雪貂、兔、大鼠、豚鼠和狍卵巢中胆碱酯酶分布的物种差异。

Species variation in the distribution of cholinesterases in the ovary of the plains viscacha, cat, ferret, rabbit, rat, guinea-pig and roe deer.

作者信息

Silver A

出版信息

Histochem J. 1978 Jan;10(1):79-102. doi: 10.1007/BF01003416.

Abstract

Sections of ovary from plains viscacha, cat, ferret, rabbit, rat, guinea-pig and roe deer have been histochemically processed to demonstrate acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) in nervous and non-nervous tissue. The effects of different reproductive states on enzyme activity were observed in some animals. AChE-containing nerves were sparse in rabbit and rat but plentiful in cat and roe deer. Nerves containing BuChE were not detectable in ferret or guinea-pig and were rare in cat. Species variations in the activity and type of enzyme were also found in non-neuronal tissues. Some blood vessels in the ovaries of guinea-pig and viscacha contained AChE. No other species showed a reaction for AChE in non-neuronal stromal tissue but BuChE was present at this site in all animals except rat. Granulosa cells reacted for AChE only in cat and rabbit while luteal cells were reactive in cat, rabbit and roe deer. Some BuChE activity was present in granulosa and or luteal cells in all species except roe deer. In rat, BuChE activity in luteal cells increased during pregnancy and the early phase of pseudopregnancy. The difficulty of assigning a function to ovarian cholinesterases is discussed.

摘要

对平原毛丝鼠、猫、雪貂、兔、大鼠、豚鼠和狍的卵巢切片进行了组织化学处理，以显示神经组织和非神经组织中的乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）。在一些动物中观察了不同生殖状态对酶活性的影响。含AChE的神经在兔和大鼠中稀疏，但在猫和狍中丰富。在雪貂或豚鼠中未检测到含BuChE的神经，在猫中也很少见。在非神经元组织中也发现了酶活性和类型的物种差异。豚鼠和毛丝鼠卵巢中的一些血管含有AChE。没有其他物种在非神经元基质组织中显示出AChE反应，但除大鼠外，所有动物在该部位都存在BuChE。颗粒细胞仅在猫和兔中对AChE有反应，而黄体细胞在猫、兔和狍中具有反应性。除狍外，所有物种的颗粒细胞和/或黄体细胞中都存在一些BuChE活性。在大鼠中，黄体细胞中的BuChE活性在妊娠和假妊娠早期增加。文中讨论了确定卵巢胆碱酯酶功能的困难。