Kreiswirth B N, Novick R P, Schlievert P M, Bergdoll M
Ann Intern Med. 1982 Jun;96(6 Pt 2):974-7. doi: 10.7326/0003-4819-96-6-974.
Thirteen isolates of Staphylococcus aureus that produce the toxic shock syndrome exotoxin were screened to identify and characterize this specific determinant and understand its role in pathogenicity. These stains belong to phage group I, are sensitive to phage 29, and are similar with respect to their resistance to cadmium, arsenate, and penicillin. These three resistances, commonly found on plasmids in many strains of S. aureus, were not plasmid-associated in 13 toxic shock strains. The cadmium and arsenate resistances were cotransferred both in transduction and in protoplast fusion; penicillin resistance was unlinked. The toxic syndrome exotoxin gene was not linked to any of these three traits. We suggest that the trait is borne by a special genetic element that acts as a heterologous chromosomal insertion and is independent of the cadmium-arsenate linkage group or the penicillinase-determinant. The genetic properties of extracellular proteins in S. aureus are reviewed as possible models for the acquisition or expression of this toxic shock antigen.
对13株产生中毒性休克综合征外毒素的金黄色葡萄球菌分离株进行了筛选，以鉴定和表征这一特定决定簇，并了解其在致病性中的作用。这些菌株属于噬菌体I组，对噬菌体29敏感，并且在对镉、砷酸盐和青霉素的抗性方面相似。这三种抗性在许多金黄色葡萄球菌菌株的质粒上常见，但在13株中毒性休克菌株中与质粒无关。镉和砷酸盐抗性在转导和原生质体融合中均能共转移；青霉素抗性不连锁。中毒综合征外毒素基因与这三个性状中的任何一个均不连锁。我们认为该性状由一种特殊的遗传元件携带，该元件作为异源染色体插入物起作用，并且独立于镉 - 砷酸盐连锁群或青霉素酶决定簇。本文综述了金黄色葡萄球菌细胞外蛋白的遗传特性，作为获取或表达这种中毒性休克抗原的可能模型。
