Ishizaka K, Ishizaka T, Okudaira H, Bazin H
J Immunol. 1978 Feb;120(2):655-60.
IgE-bearing lymphocytes were detected by immunofluorescence in the spleen of neonatal Hooded Lister strain rats within 24 hr after birth. The same cells were detected in the bone marrow as early as the 4th day after birth. Both fetal spleen and liver obtained 1 day before birth contained IgM-bearing cells but no detectable IgE-bearing cells. The proportion of IgE-bearing cells in the spleen and bone marrow increased during the neonatal period and reached an adult level within 3 to 4 weeks after birth. In adult Hooded Lister rats, IgE-bearing cells were 3 to 6% of total spleen cells and 1.5 to 2.2% of bone marrow cells. Most of the IgE-bearing cells from bone marrow cells. Most of the IgE-bearing cells from both newborn and adult animals carried IgM determinants on their surface. Capping experiments showed that epsilon chain determinants and mu chain determinants belonged to separate molecules. IgG2a-bearing lymphocytes were detected in the neonatal spleen as early as the 4th day after birth, but a significant number of these cells was not detected in the bone marrow until the 4th week. In newborn spleen the percentage of IgE-IgM double bearing cells was higher than that of IgG2a-bearing cells.
出生后24小时内，通过免疫荧光法在新生带帽利斯特品系大鼠的脾脏中检测到了携带IgE的淋巴细胞。早在出生后第4天，就在骨髓中检测到了同样的细胞。出生前1天获取的胎儿脾脏和肝脏中含有携带IgM的细胞，但未检测到携带IgE的细胞。脾脏和骨髓中携带IgE的细胞比例在新生儿期增加，并在出生后3至4周内达到成年水平。在成年带帽利斯特大鼠中，携带IgE的细胞占脾脏细胞总数的3%至6%，占骨髓细胞的1.5%至2.2%。大多数携带IgE的细胞来自骨髓细胞。新生动物和成年动物的大多数携带IgE的细胞表面都带有IgM决定簇。封帽实验表明，ε链决定簇和μ链决定簇属于不同的分子。早在出生后第4天，就在新生脾脏中检测到了携带IgG2a的淋巴细胞，但直到第4周才在骨髓中检测到大量此类细胞。在新生脾脏中，IgE-IgM双阳性细胞的百分比高于携带IgG2a的细胞。
