Sakai H, Endoh M, Tomino Y, Nomoto Y
Clin Exp Immunol. 1982 Oct;50(1):77-82.
Patients with IgA nephropathy show an emergence of IgA dominant circulating immune complexes (CIC) as well as increased levels of serum IgA and/or IgA bearing peripheral blood lymphocytes. In order to elucidate immunological aberrations responsible for the increased IgA synthesis in such patients, quantitative and qualitative analysis was performed on T alpha cells which have been recently identified as possessing IgA specific helper activity on human B cells. Three different methods were employed to quantitate T alpha cells. These methods included a rosette formation of T cells with either bovine red cells coated with the IgA fraction of anti-bovine red cell antiserum or those coated with TNP and anti-TNP IgA antibody, and an analysis of T cells combined with fluorescein conjugated human IgA myeloma protein. T alpha cells were sorted by a fluorescence activated cell sorter and co-cultured with a B cell rich fraction to evaluate whether there is a qualitative difference in IgA specific helper activity between patients and healthy adults. T alpha cells were significantly increased in patients with IgA nephropathy while there were no significant changes in patients with chronic proliferative glomerulonephritis without mesangial deposition of IgA. There was no qualitative difference in IgA specific helper activity of T alpha cells between patients and healthy adults. It is suggested that increased levels of T alpha cells in patients with IgA nephropathy may be responsible for increased synthesis of IgA in such patients.
IgA 肾病患者表现出以 IgA 为主的循环免疫复合物（CIC）出现，以及血清 IgA 水平升高和/或携带 IgA 的外周血淋巴细胞增多。为了阐明导致此类患者 IgA 合成增加的免疫异常，对最近被确定对人 B 细胞具有 IgA 特异性辅助活性的 Tα 细胞进行了定量和定性分析。采用了三种不同的方法对 Tα 细胞进行定量。这些方法包括 T 细胞与用抗牛红细胞抗血清的 IgA 部分包被的牛红细胞或用 TNP 和抗 TNP IgA 抗体包被的牛红细胞形成玫瑰花结，以及对与荧光素偶联的人 IgA 骨髓瘤蛋白结合的 T 细胞进行分析。通过荧光激活细胞分选仪对 Tα 细胞进行分选，并与富含 B 细胞的部分共培养，以评估患者和健康成年人之间 IgA 特异性辅助活性是否存在质的差异。IgA 肾病患者的 Tα 细胞显著增加，而 IgA 无系膜沉积的慢性增殖性肾小球肾炎患者则无显著变化。患者和健康成年人之间 Tα 细胞的 IgA 特异性辅助活性没有质的差异。提示 IgA 肾病患者 Tα 细胞水平升高可能是此类患者 IgA 合成增加的原因。
