Panush R S
J Clin Lab Immunol. 1982 Oct;9(1):65-70.
A simplified, sensitive assay has been devised to examine suppressor cell function in normal persons and patients with rheumatic and atopic diseases. Cultured human lymphoblastoid (IM9) cells were used as responders. Induced suppressor cells were treated initially with mitomycin (mit) C, to prevent DNA synthesis, then incubated with concanavalin (con) A in microtiter plates for 40 hr. Responder cells were added directly to suppressor cells in plates. Suppression was determined by comparing effects of con A-induced with noninduced (control) cells on responder 3H-thymidine (3HTdR) uptake. This assay is simple and conserves time, reagents, and cells and uses standardized, available responder cells. It also obviates problems of con A in cultures with responder cells and autologous or allogeneic mixed-lymphocyte types of reactions and reduces needs for blood donation. Moreover, IM9 cells proved suitable for detecting spontaneous, con A-generated, glass-adherent, or prostaglandin-secreting (indomethacin-sensitive) suppressor cells.
已设计出一种简化、灵敏的检测方法，用于检测正常人和患有风湿性疾病及特应性疾病患者的抑制细胞功能。培养的人淋巴母细胞样（IM9）细胞用作反应细胞。诱导的抑制细胞先用丝裂霉素（mit）C处理，以防止DNA合成，然后在微量滴定板中与伴刀豆球蛋白（con）A一起孵育40小时。将反应细胞直接加入平板中的抑制细胞中。通过比较伴刀豆球蛋白A诱导的细胞与未诱导（对照）细胞对反应细胞3H-胸腺嘧啶核苷（3HTdR）摄取的影响来确定抑制作用。该检测方法简单，节省时间、试剂和细胞，并使用标准化的、可得的反应细胞。它还避免了在反应细胞培养以及自体或同种异体混合淋巴细胞类型反应中伴刀豆球蛋白A的问题，并减少了献血需求。此外，IM9细胞被证明适用于检测自发的、伴刀豆球蛋白A产生的、玻璃黏附的或分泌前列腺素的（对吲哚美辛敏感的）抑制细胞。
