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水生甲虫(Asellus aquaticus (L.))中葡萄糖磷酸异构酶(GPI)和磷酸葡萄糖变位酶(PGM)多态性的描述与遗传学研究

Description and genetics of glucose phosphate isomerase (GPI) and phosphoglucomutase (PGM) polymorphisms in Asellus aquaticus (L.).

作者信息

Verspoor E

出版信息

Biochem Genet. 1982 Oct;20(9-10):891-905. doi: 10.1007/BF00484067.

Abstract

Analysis of Western European populations of Asellus aquaticus uncovered 10 electrophoretic phenotypes of glucose phosphate isomerase (GPI) and 7 of phosphoglucomutase (PGM). Breeding studies indicate that the variation is controlled by codominant alleles at two autosomal loci. Genotype frequencies in the two sexes do not differ significantly, mating between genotypes is random, and no structural linkage is detectable between the two loci. PGM shows nongenetic, "secondary" banding, particularly in animals stored at -20 degrees C prior to electrophoresis. This secondary banding confounds the identification of the genetic variation but can be controlled by the reducing agent 2-mercaptoethanol.

摘要

对西欧普通钩虾种群的分析发现,葡萄糖磷酸异构酶(GPI)有10种电泳表型,磷酸葡萄糖变位酶(PGM)有7种电泳表型。育种研究表明,这种变异受两个常染色体位点上的共显性等位基因控制。两性的基因型频率没有显著差异,基因型之间的交配是随机的,并且在这两个位点之间未检测到结构连锁。PGM显示出非遗传的“二级”条带,特别是在电泳前保存在-20摄氏度的动物中。这种二级条带混淆了遗传变异的识别,但可以通过还原剂2-巯基乙醇来控制。

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