Tomonari K
J Immunol. 1980 Mar;124(3):1111-21.
In the course of the culture of an autologous mixed lymphocyte reaction (AMLR), T cells proliferated in response to autologous non-T cells, and differentiated to cytotoxic T cells (AMLR killers). DNA synthesis was necessary to generate AMLR killers, as the elimination of autoreactive proliferating cells with BUdR and UV light completely abrogated AMLR killer cytolysis. Amlr killers lysed various lymphoid cell lines, including autologous B cell lines, autologous or allogeneic mitogen blasts stimulated by Con A, PHA, or pokeweed mitogen, variious nonlymphoid cell lines derived from human, mouse, or rat, and weakly normal autologous or allogeneic non-T cells. KMT-17, methylcholanthrene-induced rat fibrosarcoma, was the only resistant cell line to have been tested. AMLR killers had characteristics similar to NK cells, Major histocompatibility antigens were not the target antigens for AMLR killers. AMLR killers distinguished the blasts stimulated by alloantigens as self from the blasts stimulated by mitogens as non-self.
在自体混合淋巴细胞反应（AMLR）培养过程中，T细胞对自体非T细胞产生增殖反应，并分化为细胞毒性T细胞（AMLR杀伤细胞）。DNA合成对于产生AMLR杀伤细胞是必要的，因为用5-溴脱氧尿苷（BUdR）和紫外线消除自身反应性增殖细胞可完全消除AMLR杀伤细胞的细胞溶解作用。AMLR杀伤细胞可裂解多种淋巴细胞系，包括自体B细胞系、由刀豆蛋白A（Con A）、植物血凝素（PHA）或商陆有丝分裂原刺激的自体或同种异体有丝分裂原母细胞、源自人、小鼠或大鼠的各种非淋巴细胞系，以及正常自体或同种异体非T细胞，但作用较弱。KMT-17，即甲基胆蒽诱导的大鼠纤维肉瘤，是唯一经测试的耐药细胞系。AMLR杀伤细胞具有与自然杀伤（NK）细胞相似的特征，主要组织相容性抗原不是AMLR杀伤细胞的靶抗原。AMLR杀伤细胞将同种异体抗原刺激的母细胞识别为自身，而将有丝分裂原刺激的母细胞识别为非自身。
