Tomonari K, Wakisaka A, Aizawa M
J Immunol. 1980 Oct;125(4):1596-600.
Human T cells were separated with neuraminidase-treated autologous erythrocytes into auto-rosette-forming cells (ARFC) and non-ARFC (N-ARFC). These 2 subsets were examined for reactivity to autologous non-T cells. When N-ARFC were stimulated by autologous non-T cells, blastogenesis was weak; ARFC, however, proliferated vigorously. ARFC were revealed to be the major subset that responded and proliferated in autologous mixed lymphocyte reaction (AMLR). AMLR-primed cells were restimulated by autologous or allogeneic non-T cells to study self recognition in relation to HLA complex. Self recognition by AMLR-primed cells was closely related to HLA complex, and absolute identity of HLA complex was necessary to recognize allogeneic cells as self. In addition, in respect to the responder cell difference between AMLR and allogeneic MLR, the reactivity of 2 kinds of cells (i.e., AMLR-primed cells and alloreactive cells) strongly suggested that autoreactive T cells were distinguished from alloreactive T cells.
用人神经氨酸酶处理过的自体红细胞将人T细胞分离为自身玫瑰花结形成细胞（ARFC）和非ARFC（N - ARFC）。检测这两个亚群对自体非T细胞的反应性。当N - ARFC受到自体非T细胞刺激时，细胞增殖反应较弱；然而，ARFC则强烈增殖。ARFC被发现是自体混合淋巴细胞反应（AMLR）中发生反应并增殖的主要亚群。用自体或同种异体非T细胞再次刺激AMLR致敏细胞，以研究与HLA复合体相关的自身识别。AMLR致敏细胞的自身识别与HLA复合体密切相关，并且HLA复合体的绝对一致性对于将同种异体细胞识别为自身细胞是必要的。此外，关于AMLR和同种异体混合淋巴细胞反应（MLR）中反应细胞的差异，两种细胞（即AMLR致敏细胞和同种异体反应性细胞）的反应性强烈表明自身反应性T细胞与同种异体反应性T细胞是有区别的。
