人肾组织中酸性糖胺聚糖的年龄依赖性分布

Age-dependent distribution of acidic glycosaminoglycans in human kidney tissue.

作者信息

Murata K, Horiuchi Y

出版信息

Nephron. 1978;20(2):111-8. doi: 10.1159/000181203.

Abstract

Acidic glycosaminoglycans (AGAG) of human kidneys prepared from subjects of different ages were purified and determined by an enzymic assay with chondroitinases. The resulting disaccharide units and the undigested AGAG after digesting with the enzymes were identified by paper chromatography, electrophoresis and thin-layer chromatography. The age-dependent changes were: heparan sulfates accounted for 43-74% of total AGAG and the proportion to total AGAG appeared to decrease with advancing age. Dermatan sulfate remained without change. The proportion of hyaluronic acid appeared to decrease at 19-21 years and increased again with aging. The ratio of the 4-type chondroitin sulfate to the 6-type decreased with advancing age.

摘要

从不同年龄段受试者制备的人肾酸性糖胺聚糖（AGAG），经用软骨素酶进行酶法测定予以纯化和测定。酶解后得到的二糖单位和未消化的AGAG，通过纸色谱法、电泳法和薄层色谱法进行鉴定。年龄依赖性变化如下：硫酸乙酰肝素占总AGAG的43%-74%，且占总AGAG的比例似乎随着年龄的增长而降低。硫酸皮肤素保持不变。透明质酸的比例在19-21岁时似乎降低，之后随着年龄增长再次升高。硫酸软骨素4型与6型的比例随着年龄的增长而降低。