Lemm G, Salzer K, Warnatz H
Clin Exp Immunol. 1983 May;52(2):250-8.
Patients with acute hepatitis B and HBV-induced chronic hepatitis as well as normal control persons participated in the study. Hepatitis patients of both groups have decreased OKT4+/OKT8+T cell ratios due to an percental increase of OKT8+T cells in peripheral blood compared to the data of controls. Lymphocyte cultures of chronic hepatitis patients show reduced DNA synthesis after stimulation by allogeneic non-T cells, PHA, Con A and PWM. PWM-induced immunoglobulin secretion by B cells, determined by means of a reverse haemolytic plaque assay (RHPA) and a solid phase ELISA, showed comparable results in hepatitis B patients and controls. The AMLR, which is thought to reflect an autologous immunoregulatory phenomenon, is slightly impaired in cultures of hepatitis B patients in comparison to controls. Con A-induced suppressor cell activity on T cell reactions is decreased in hepatitis, whereas suppressor cell activity on B cell activation is within the same range as in cultures of controls. It is concluded from these data, that suppressor cell activity on T cell function is impaired in hepatitis B, whereas B cell functions and suppressor cell activity on B cell function are in the normal range. The results with the functional assays and the finding of increased proportions of OKT8+T cells in hepatitis B are considered to reflect properties of different T cell subpopulations, responsible for different immunoregulatory functions.
急性乙型肝炎和乙肝病毒诱导的慢性肝炎患者以及正常对照者参与了该研究。与对照组数据相比，两组肝炎患者外周血中OKT8 + T细胞百分比增加，导致OKT4 + / OKT8 + T细胞比值降低。慢性肝炎患者的淋巴细胞培养物在经同种异体非T细胞、PHA、Con A和PWM刺激后，DNA合成减少。通过反向溶血空斑试验（RHPA）和固相ELISA测定，PWM诱导的B细胞免疫球蛋白分泌在乙肝患者和对照组中显示出可比的结果。被认为反映自体免疫调节现象的AMLR在乙肝患者培养物中与对照组相比略有受损。肝炎中Con A诱导的对T细胞反应的抑制细胞活性降低，而对B细胞活化的抑制细胞活性与对照组培养物中的处于相同范围。从这些数据得出结论，乙肝中对T细胞功能的抑制细胞活性受损，而B细胞功能以及对B细胞功能的抑制细胞活性处于正常范围。功能测定的结果以及乙肝中OKT8 + T细胞比例增加的发现被认为反映了负责不同免疫调节功能的不同T细胞亚群的特性。
