Kashio T, Hotta R, Kakumu S
Clin Exp Immunol. 1981 Jun;44(3):459-66.
We investigated lymphocyte suppressor cell activity in 53 patients with acute and chronic liver diseases. Suppressor cells were generated by preincubation of peripheral blood mononuclear cells (PBM) with concanavalin A (Con A) for 48 hr. Suppressor cell activity was evaluated by inhibition of Con A-stimulated blast transformation and by inhibition of pokeweed mitogen-induced immunoglobulin (Ig) synthesis of fresh allogeneic normal PBM in the second-set cultures. Of 29 patients with chronic active liver diseases (CALD), defective suppressor cell activities were observed in eight cases (28%) for Ig synthesis and 16 cases (55%) for blast transformation study. The suppressor cell activities were decreased in two (22%) of nine cases with chronic persistent hepatitis and one (17%) of six cases with inactive cirrhosis for both Ig synthesis and blast transformation. In contrast, suppressor activities were inducible in all nine patients with acute viral hepatitis. The histocompatibility antigen DR4 was significantly increased in CALD patients, but there was no correlation between this antigen and suppressor cell activity. These findings suggest that altered lymphocyte suppressor cells in patients with CALD may contribute to the continuing liver cell injury in this disease.
我们研究了53例急慢性肝病患者的淋巴细胞抑制细胞活性。通过将外周血单个核细胞（PBM）与刀豆蛋白A（Con A）预孵育48小时来产生抑制细胞。在二次培养中，通过抑制Con A刺激的母细胞转化以及抑制新鲜同种异体正常PBM的商陆有丝分裂原诱导的免疫球蛋白（Ig）合成来评估抑制细胞活性。在29例慢性活动性肝病（CALD）患者中，8例（28%）的Ig合成和16例（55%）的母细胞转化研究观察到抑制细胞活性缺陷。对于Ig合成和母细胞转化，9例慢性持续性肝炎患者中有2例（22%）、6例非活动性肝硬化患者中有1例（17%）的抑制细胞活性降低。相比之下，所有9例急性病毒性肝炎患者的抑制活性均可诱导。CALD患者的组织相容性抗原DR4显著增加，但该抗原与抑制细胞活性之间无相关性。这些发现表明，CALD患者淋巴细胞抑制细胞的改变可能导致该疾病中持续的肝细胞损伤。
