一株依赖氨甲酰天冬氨酸来抵抗5-氟尿嘧啶的鼠伤寒沙门氏菌突变体在泛醌生物合成中受到特异性影响。
A Salmonella typhimurium mutant dependent upon carbamyl aspartate for resistance to 5-fluorouracil is specifically affected in ubiquinone biosynthesis.
作者信息
Zak V L, Kelln R A
出版信息
J Bacteriol. 1981 Feb;145(2):1095-8. doi: 10.1128/jb.145.2.1095-1098.1981.
Abstract
The isolation and properties of a mutant dependent upon exogenous carbamyl aspartate for resistance to 5-fluorouracil are described. The mutant was deficient in the synthesis of ubiquinone and accumulated a quinone provisionally identified as the ubiquinone precursor 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. The mutation resulted in an alteration in the regulation of synthesis of enzymes involved in de novo pyrimidine biosynthesis but did not establish a functional block in dihydroorotate dehydrogenase activity in vivo. Conditional resistance to 5-fluorouracil apparently occurred through an inhibition of the conversion of the analog to the nucleotide level.
摘要
本文描述了一种依赖外源性氨甲酰天冬氨酸来抵抗5-氟尿嘧啶的突变体的分离及其特性。该突变体在泛醌合成方面存在缺陷，并积累了一种暂时鉴定为泛醌前体2-辛戊烯基-3-甲基-6-甲氧基-1,4-苯醌的醌类物质。该突变导致从头嘧啶生物合成中相关酶的合成调控发生改变，但在体内二氢乳清酸脱氢酶活性方面并未形成功能性阻断。对5-氟尿嘧啶的条件抗性显然是通过抑制该类似物向核苷酸水平的转化而发生的。
相似文献
1
A Salmonella typhimurium mutant dependent upon carbamyl aspartate for resistance to 5-fluorouracil is specifically affected in ubiquinone biosynthesis.一株依赖氨甲酰天冬氨酸来抵抗5-氟尿嘧啶的鼠伤寒沙门氏菌突变体在泛醌生物合成中受到特异性影响。
J Bacteriol. 1981 Feb;145(2):1095-8. doi: 10.1128/jb.145.2.1095-1098.1981.
2
Apparent involvement of purines in the control of expression of Salmonella typhimurium pyr genes: analysis of a leaky guaB mutant resistant to pyrimidine analogs.嘌呤明显参与鼠伤寒沙门氏菌pyr基因表达的调控：对一种对嘧啶类似物有抗性的渗漏guaB突变体的分析。
J Bacteriol. 1979 Jun;138(3):731-8. doi: 10.1128/jb.138.3.731-738.1979.
3
RNA polymerase involvement in the regulation of expression of Salmonella typhimurium pyr genes. Isolation and characterization of a fluorouracil-resistant mutant with high, constitutive expression of the pyrB and pyrE genes due to a mutation in rpoBC.RNA聚合酶参与鼠伤寒沙门氏菌pyr基因表达的调控。由于rpoBC发生突变，分离并鉴定了一株对氟尿嘧啶耐药的突变体，该突变体中pyrB和pyrE基因呈高组成型表达。
EMBO J. 1982;1(1):69-74. doi: 10.1002/j.1460-2075.1982.tb01126.x.
4
Isolation and partial characterization of regulatory mutants of the pyrimidine pathway in Salmonella typhimurium.鼠伤寒沙门氏菌嘧啶途径调控突变体的分离与部分特性分析。
J Bacteriol. 1972 Mar;109(3):1085-96. doi: 10.1128/jb.109.3.1085-1096.1972.
5
Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium.鼠伤寒沙门氏菌对嘧啶和嘧啶核苷的代谢
J Bacteriol. 1972 Apr;110(1):219-28. doi: 10.1128/jb.110.1.219-228.1972.
6
A mutation in Salmonella typhimurium imparting conditional resistance to 5-fluorouracil and a bioenergetic defect: mapping of cad.鼠伤寒沙门氏菌中赋予对5-氟尿嘧啶条件抗性和生物能量缺陷的突变：cad的定位
Mol Gen Genet. 1980;179(3):677-81. doi: 10.1007/BF00271757.
7
Toxicity of the pyrimidine biosynthetic pathway intermediate carbamyl aspartate in Salmonella typhimurium.鼠伤寒沙门氏菌中嘧啶生物合成途径中间体氨甲酰天冬氨酸的毒性
J Bacteriol. 1985 Aug;163(2):500-5. doi: 10.1128/jb.163.2.500-505.1985.
8
Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone.大肠杆菌K-12积累双醌前体2-辛戊烯基-6-甲氧基-1,4-苯醌和2-辛戊烯基-3-甲基-6-甲氧基-1,4-苯醌的突变菌株的表征及遗传分析
J Bacteriol. 1971 Mar;105(3):769-78. doi: 10.1128/jb.105.3.769-778.1971.
9
Isolation and mapping of a uracil-sensitive mutant of Salmonella typhimurium.鼠伤寒沙门氏菌尿嘧啶敏感突变体的分离与定位
Mol Gen Genet. 1982;185(3):513-4. doi: 10.1007/BF00334151.
10
Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis.大肠杆菌K-12泛醌生物合成最终反应受阻的突变体：特性与遗传分析。
J Bacteriol. 1972 Jan;109(1):134-9. doi: 10.1128/jb.109.1.134-139.1972.
引用本文的文献
1
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
2
Construction and use of pyr::lac fusion strains to study regulation of pyrimidine biosynthesis in Salmonella typhimurium.构建和使用pyr::lac融合菌株以研究鼠伤寒沙门氏菌中嘧啶生物合成的调控。
Mol Gen Genet. 1983;189(3):463-70. doi: 10.1007/BF00325910.
3
Toxicity of the pyrimidine biosynthetic pathway intermediate carbamyl aspartate in Salmonella typhimurium.鼠伤寒沙门氏菌中嘧啶生物合成途径中间体氨甲酰天冬氨酸的毒性
J Bacteriol. 1985 Aug;163(2):500-5. doi: 10.1128/jb.163.2.500-505.1985.
4
Redox regulation of the genes for cobinamide biosynthesis in Salmonella typhimurium.鼠伤寒沙门氏菌中钴胺酰胺生物合成基因的氧化还原调节
J Bacteriol. 1989 Dec;171(12):6734-9. doi: 10.1128/jb.171.12.6734-6739.1989.
本文引用的文献
1
ENZYMES OF THE PYRIMIDINE PATHWAY IN ESCHERICHIA COLI. II. INTRACELLULAR LOCALIZATION AND PROPERTIES OF DIHYDROOROTIC DEHYDROGENASE.大肠杆菌中嘧啶途径的酶。II. 二氢乳清酸脱氢酶的细胞内定位及特性
J Bacteriol. 1964 Jul;88(1):105-10. doi: 10.1128/jb.88.1.105-110.1964.
2
A mutation in Salmonella typhimurium imparting conditional resistance to 5-fluorouracil and a bioenergetic defect: mapping of cad.鼠伤寒沙门氏菌中赋予对5-氟尿嘧啶条件抗性和生物能量缺陷的突变：cad的定位
Mol Gen Genet. 1980;179(3):677-81. doi: 10.1007/BF00271757.
3
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
4
Pyrimidine pools and macromolecular composition of pyrimidine-limited Escherichia coli.嘧啶受限的大肠杆菌的嘧啶库与大分子组成
J Bacteriol. 1970 Apr;102(1):118-23. doi: 10.1128/jb.102.1.118-123.1970.
5
Dihydroorotate-ubiquinone reductase complex of Escherichia coli B.
J Biol Chem. 1968 Jun 10;243(11):2963-8.
6
Some improved methods in P22 transduction.P22转导中的一些改进方法。
Genetics. 1974 Apr;76(4):625-31. doi: 10.1093/genetics/76.4.625.
7
Structural genes for catalytic and regulatory subunits of aspartate transcarbamylase.
J Mol Biol. 1973 May 25;76(3):363-78. doi: 10.1016/0022-2836(73)90510-x.
8
Dihydro-orotate dehydrogenase of Escherichia coli K12: effects of triton X-100 and phospholipids.
Biochim Biophys Acta. 1973 Apr 12;302(2):205-15. doi: 10.1016/0005-2744(73)90149-6.
9
10
Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone.大肠杆菌K-12积累双醌前体2-辛戊烯基-6-甲氧基-1,4-苯醌和2-辛戊烯基-3-甲基-6-甲氧基-1,4-苯醌的突变菌株的表征及遗传分析
J Bacteriol. 1971 Mar;105(3):769-78. doi: 10.1128/jb.105.3.769-778.1971.
Zotero 插件