Spierer P, Spierer A, Bender W, Hogness D S
J Mol Biol. 1983 Jul 25;168(1):35-50. doi: 10.1016/s0022-2836(83)80321-0.
We have used a set of overlapping cloned segments defining a 315 kb (X 10(3) base-pairs) region of Drosophila melanogaster chromosomal DNA to map the sequences associated with the polytene band-interbands (chromomeric units) and with the lethal complementation groups contained within this region. The molecular map positions of the 13 +/- 1 chromomeric units from the 87D5-6 to 87E5, 6 region of the third chromosome were determined by in situ hybridization of selected segments to the polytene chromosomes. The length of the largest chromomeric unit within the 315 kb region is approximately 160 kb, while that for the smallest is less than 7 kb and may be as short as 3 kb. By mapping the breakpoints of deletions within the 315 kb region, we have located its 12 lethal complementation groups, which include the genes coding for acetylcholinesterase (Ace) and xanthine dehydrogenase (rosy). Comparison of the two molecular maps indicates a one-to-one topographical correlation between the genetic and chromomeric units.
我们使用了一组重叠的克隆片段，这些片段定义了果蝇黑腹染色体DNA的一个315 kb（×10³碱基对）区域，以绘制与多线带间带（染色粒单位）以及该区域内所含致死互补群相关的序列图谱。通过将选定片段与多线染色体进行原位杂交，确定了第三染色体87D5 - 6至87E5、6区域中13 ± 1个染色粒单位的分子图谱位置。在315 kb区域内，最大染色粒单位的长度约为160 kb，而最小的则小于7 kb，可能短至3 kb。通过绘制315 kb区域内缺失的断点图谱，我们定位了其12个致死互补群，其中包括编码乙酰胆碱酯酶（Ace）和黄嘌呤脱氢酶（rosy）的基因。两种分子图谱的比较表明，遗传单位和染色粒单位之间存在一对一的地形相关性。
