秋水仙碱对家族性地中海热免疫调节异常的影响。

Effect of colchicine on immunoregulatory abnormalities in familial Mediterranean fever.

作者信息

Schlesinger M, Ilfeld D, Handzel Z T, Altman Y, Kuperman O, Levin S, Bibi C, Netzer L, Trainin N

出版信息

Clin Exp Immunol. 1983 Oct;54(1):73-9.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1536202/

Abstract

The effect of colchicine on immunoregulatory T lymphocytes in children with familial Mediterranean fever (FMF) was studied. Concanavalin A (Con A)-induced suppressor cell function was significantly (P less than 0.0001) decreased in five untreated FMF patients (15 +/- 3%, mean +/- s.e.) as compared to six age matched paediatric controls (46 +/- 3%) and eight healthy adults (49 +/- 4%). When the five untreated FMF patients' mononuclear cells were pre-incubated in vitro with Con A plus 10(-5) M colchicine, their suppressor cell function was significantly increased (52 +/- 10%, P less than 0.01). Similarly, oral colchicine treatment (0.5 mg twice daily) significantly (P = 0.02) increased the five FMF patients' Con A-induced suppressor cell function to levels (34 +/- 6%) that were not significantly (P greater than 0.05) different than the paediatric controls or the healthy adults. The percentage of OKT8+ cells (but not OKT3+ or OKT4+ cells) was significantly (P less than 0.0001) decreased in 10 untreated FMF patients (16.0 +/- 0.9) as compared to 10 paediatric controls (27.6 +/- 2) or 10 healthy adults (25.7 +/- 0.6). The 10 untreated FMF patients had a significant (P less than 0.002) increase in the OKT4/OKT8 ratio (2.41 +/- 0.13) as compared to 10 FMF patients treated with 0.5 mg twice daily of colchicine (1.81 +/- 0.08), 10 pediatric controls (1.47 +/- 0.2), or 10 healthy adults (1.78 +/- 0.11). Colchicine appears to have corrected the FMF patients' elevated OKT4/OKT8 ratio by both decreasing the percentage of OKT4+ cells and increasing (but only partially correcting) the percentage of OKT8+ cells. Thus FMF patients have a suppressor cell deficiency in which colchicine treatment corrects their deficiency of Con A-induced suppressor cell function and their elevated OKT4/OKT8 ratio. This raises the possibility that colchicine might be potentially useful as an immunomodulating drug in treating patients with autoimmune or allergic diseases associated with a suppressor cell deficiency.

摘要

研究了秋水仙碱对家族性地中海热（FMF）患儿免疫调节性T淋巴细胞的影响。与6名年龄匹配的儿科对照（46±3%）和8名健康成年人（49±4%）相比，5名未经治疗的FMF患者（15±3%，平均值±标准误）中，伴刀豆球蛋白A（Con A）诱导的抑制细胞功能显著降低（P<0.0001）。当5名未经治疗的FMF患者的单核细胞在体外与Con A加10⁻⁵ M秋水仙碱预孵育时，其抑制细胞功能显著增强（52±10%，P<0.01）。同样，口服秋水仙碱治疗（每日两次，每次0.5 mg）显著（P = 0.02）提高了5名FMF患者Con A诱导的抑制细胞功能，使其达到与儿科对照或健康成年人无显著差异（P>0.05）的水平（34±6%）。与10名儿科对照（27.6±2）或10名健康成年人（25.7±0.6）相比，10名未经治疗的FMF患者中OKT8⁺细胞的百分比显著降低（P<0.0001）（16.0±0.9）。与10名每日两次服用0.5 mg秋水仙碱治疗的FMF患者（1.81±0.08）、10名儿科对照（1.47±0.2）或10名健康成年人（1.78±0.11）相比，10名未经治疗的FMF患者的OKT4/OKT8比值显著升高（P<0.002）（2.41±0.13）。秋水仙碱似乎通过降低OKT4⁺细胞百分比和增加（但仅部分纠正）OKT8⁺细胞百分比来纠正FMF患者升高的OKT4/OKT8比值。因此，FMF患者存在抑制细胞缺陷，秋水仙碱治疗可纠正其Con A诱导的抑制细胞功能缺陷及其升高的OKT4/OKT8比值。这增加了秋水仙碱作为免疫调节药物治疗与抑制细胞缺陷相关的自身免疫性或过敏性疾病患者的潜在可能性。