Petersen J, Andersen V, Ingemann-Hansen T, Halkjaer-Kristensen J, Wiik A, Thyssen H
Scand J Rheumatol. 1983;12(3):299-304. doi: 10.3109/03009748309098553.
The influence of synovial fluid and blood monocytes on autologous lymphocyte proliferation in vitro was investigated in patients with rheumatoid arthritis (RA) and traumatic synovitis (TS). In cultures stimulated with PHA, PWM and ConA, thymidine incorporation by RA blood mononuclear cells (BMC) increased considerably in most cases after addition of 10% or 30% synovial fluid monocytes and a similar effect was seen after stimulation with Candida extract or PPD. A comparable helper activity was observed after addition of synovial fluid monocytes to monocyte-depleted blood cells in RA. The augmenting effect of synovial fluid monocytes was likewise demonstrated in TS patients. Synovial fluid mononuclear cells (SMC) from RA and TS patients displayed low proliferative responses to PHA and ConA in comparison with BMC, while the responses to microbial antigens were similar or greater. The proliferative activity of SMC or of monocyte-depleted synovial fluid cells after polyclonal and antigenic stimulation was not affected by addition of blood monocytes. This was observed in both RA and TS patients. It is concluded that the regulatory properties of synovial fluid monocytes in RA and TS do not explain the low proliferative responses of SMC to polyclonal activators.
研究了类风湿性关节炎（RA）和创伤性滑膜炎（TS）患者滑液单核细胞和血液单核细胞对体外自体淋巴细胞增殖的影响。在用PHA、PWM和ConA刺激的培养物中，添加10%或30%的滑液单核细胞后，大多数情况下RA血液单核细胞（BMC）的胸腺嘧啶核苷掺入量显著增加，在用念珠菌提取物或PPD刺激后也观察到类似效果。在RA中，向单核细胞耗竭的血细胞中添加滑液单核细胞后观察到了类似的辅助活性。滑液单核细胞的增强作用在TS患者中同样得到证实。与BMC相比，RA和TS患者的滑液单核细胞（SMC）对PHA和ConA的增殖反应较低，而对微生物抗原的反应相似或更强。多克隆和抗原刺激后，SMC或单核细胞耗竭的滑液细胞的增殖活性不受血液单核细胞添加的影响。在RA和TS患者中均观察到这一点。得出的结论是，RA和TS中滑液单核细胞的调节特性并不能解释SMC对多克隆激活剂的低增殖反应。
