Petersen J, Halkjaer-Kristensen J, Ingemann-Hansen T
Scand J Rheumatol. 1984;13(3):265-72. doi: 10.3109/03009748409100396.
The regulatory role of synovial fluid monocytes/macrophages from patients with rheumatoid arthritis in terms of B lymphocyte activation was evaluated by a reverse haemolytic plaque-forming cell (PFC) assay. Macrophage-depleted blood mononuclear cells (BMC) failed to respond to pokeweed mitogen (PWM). With autologous synovial fluid macrophages added, the PFC responses of macrophage-depleted BMC increased, and optimal concentration for full restoration of the PFC responses ranged from 8 to 35%. Synovial fluid mononuclear cells (SMC) as well as macrophage-depleted SMC were not able to respond to PWM. Addition of irradiated autologous blood macrophages to SMC did not increase the SMC PFC responses. It is concluded that the regulatory properties of synovial fluid macrophages do not explain the low PFC response of SMC to PWM.
通过反向溶血空斑形成细胞（PFC）试验评估类风湿性关节炎患者滑膜液单核细胞/巨噬细胞在B淋巴细胞激活方面的调节作用。去除巨噬细胞的血液单核细胞（BMC）对商陆有丝分裂原（PWM）无反应。加入自体滑膜液巨噬细胞后，去除巨噬细胞的BMC的PFC反应增加，PFC反应完全恢复的最佳浓度范围为8%至35%。滑膜液单核细胞（SMC）以及去除巨噬细胞的SMC对PWM均无反应。向SMC中加入经辐照的自体血液巨噬细胞并未增加SMC的PFC反应。得出的结论是，滑膜液巨噬细胞的调节特性并不能解释SMC对PWM的低PFC反应。
