Orberg J, Baer E, Hiltner A
Connect Tissue Res. 1983;11(4):285-97. doi: 10.3109/03008208309004861.
The characteristic extinction pattern which is observed when the submucosa is viewed in the optical polarizing microscope has been analyzed in terms of the configuration and orientation of the 4 micron diameter collagen fibers. It is shown that the observed polarization effects are produced by periodic variations in orientation of fully birefringent fibers. The fiber configuration required to produce the observed polarization effects is a tilted wave configuration with a crimp period of approximately 20 micron. In the model, the tilted waveform fibers are crimped in register and form parallel arrays. The arrays are oriented in layers at approximately +30 degrees and -30 degrees to the longitudinal direction and are mirror images of each other. Analysis of the extinction pattern shows that the model satisfactorily accounts for the observed polarization effects at several different angles of the crossed polaroids. The calculated strain necessary to straighten the wavy fibers of the model correlates well with the observed strain to uncrimp the collagen fibers in the intestine. This suggests that the initial response to stress is gradual uncrimping of the collagen fibers, and concurrently, a decrease in the angle between biaxially oriented fibers, rather than extension of the straight fibers.
在光学偏振显微镜下观察黏膜下层时所观察到的特征性消光模式，已根据直径为4微米的胶原纤维的形态和取向进行了分析。结果表明，观察到的偏振效应是由完全双折射纤维取向的周期性变化产生的。产生观察到的偏振效应所需的纤维形态是一种倾斜波形态，其卷曲周期约为20微米。在该模型中，倾斜波形纤维同步卷曲并形成平行排列。这些排列在与纵向方向大约呈+30度和-30度的层面上取向，且彼此为镜像。对消光模式的分析表明，该模型令人满意地解释了在几个不同的偏振片交叉角度下观察到的偏振效应。计算得出的使模型中波浪状纤维变直所需的应变与观察到的使肠道中胶原纤维解卷曲的应变密切相关。这表明对应力的初始反应是胶原纤维逐渐解卷曲，同时，双轴取向纤维之间的角度减小，而不是直纤维的伸展。
