Coulam C B, Silverfield J C, Kazmar R E, Fathman C G
Am J Reprod Immunol (1980). 1983 Sep;4(2):88-90. doi: 10.1111/j.1600-0897.1983.tb00259.x.
Many observations suggest that cellular immunity is depressed during gestation. To define alterations in subsets of circulating lymphocytes during pregnancy, we compared the percentages of total T cells, T helper cells, and T suppressor cells in the peripheral blood of 31 pregnant women at various stages of gestation with those in that of nonpregnant controls. Control studies consisted of five women ingesting oral contraceptives and five women menstruating spontaneously. Isolated peripheral blood mononuclear cells were reacted with fluoresceinated monoclonal antibodies that were specific for Leu-2 cells, Leu-3 cells, and Leu-4 cells and that were subsequently analyzed on a fluorescence-activated cell sorter. No variation in the percentage of Leu-2, Leu-3, or Leu-4 cells was observed during the menstrual cycle or oral contraceptive cycle. No differences were seen when T-cell subsets at various stages of gestation were compared with the corresponding T-cell subset in nonpregnant controls. The data suggest that pregnancy does not alter the percentage of T-cell subsets in the peripheral blood.
许多观察结果表明，孕期细胞免疫功能受到抑制。为了明确孕期循环淋巴细胞亚群的变化，我们比较了31名处于不同孕期阶段的孕妇外周血中总T细胞、辅助性T细胞和抑制性T细胞的百分比与非孕对照组的相应百分比。对照研究包括5名服用口服避孕药的女性和5名自然月经周期的女性。分离出的外周血单个核细胞与针对Leu - 2细胞、Leu - 3细胞和Leu - 4细胞的荧光单克隆抗体反应，随后在荧光激活细胞分选仪上进行分析。在月经周期或口服避孕药周期中，未观察到Leu - 2、Leu - 3或Leu - 4细胞百分比的变化。当将孕期不同阶段的T细胞亚群与非孕对照组的相应T细胞亚群进行比较时，未发现差异。数据表明，妊娠不会改变外周血中T细胞亚群的百分比。
