BGI-Shenzhen, Shenzhen, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Clin Transl Med. 2022 May;12(5):e821. doi: 10.1002/ctm2.821.
During pregnancy, mother-child interactions trigger a variety of subtle changes in the maternal body, which may be reflected in the status of peripheral blood mononuclear cells (PBMCs). Although these cells are easy to access and monitor, a PBMC atlas for pregnant women has not yet been constructed.
We applied single-cell RNA sequencing (scRNA-seq) to profile 198,356 PBMCs derived from 136 pregnant women (gestation weeks 6 to 40) and a control cohort. We also used scRNA-seq data to establish a transcriptomic clock and thereby predicted the gestational age of normal pregnancy.
We identified reconfiguration of the peripheral immune cell phenotype during pregnancy, including interferon-stimulated gene upregulation, activation of RNA splicing-related pathways and immune activity of cell subpopulations. We also developed a cell-type-specific model to predict gestational age of normal pregnancy.
We constructed a single-cell atlas of PBMCs in pregnant women spanning the entire gestation period, which should help improve our understanding of PBMC composition turnover in pregnant women.
在妊娠期间，母婴互动会引发母体身体的多种微妙变化，这些变化可能反映在外周血单个核细胞（PBMC）的状态中。尽管这些细胞很容易获取和监测，但尚未构建针对孕妇的 PBMC 图谱。
我们应用单细胞 RNA 测序（scRNA-seq）对来自 136 名孕妇（妊娠 6 至 40 周）和对照组的 198356 个 PBMC 进行了分析。我们还使用 scRNA-seq 数据建立了转录组钟，并由此预测了正常妊娠的胎龄。
我们发现妊娠期间外周免疫细胞表型发生了重新配置，包括干扰素刺激基因的上调、与 RNA 剪接相关途径的激活以及细胞亚群的免疫活性。我们还开发了一种细胞类型特异性模型来预测正常妊娠的胎龄。
我们构建了一个涵盖整个妊娠期间的孕妇 PBMC 的单细胞图谱，这应该有助于我们更好地理解孕妇 PBMC 组成的变化。
