Pavia C, Siiteri P K, Perlman J D, Stites D P
J Reprod Immunol. 1979 Jan-Feb;1(1):33-8. doi: 10.1016/0165-0378(79)90027-5.
Investigations have been carried out on the action of several steroid hormones on lymphocyte functions in inbred strains of mice. The recognitive, proliferative and effector phases of allogeneic cell interactions in vitro were assessed using a mixed lymphocyte reaction (MLR) and cell-mediated lympholysis (CML). In MLR containing Balb/c (responder) and C57bl/6 (stimulator) splenocytes DNA synthesis was markedly reduced in the presence of progesterone, cortisol or estradiol. In CML, progesterone and estradiol (1-5 microgram/ml) blocked in vitro generation of cytotoxic lymphocytes, while cultures with cortisol were partially inhibited. None of these hormones suppressed the cytotoxic activity of previously sensitized effector cells generated in vitro. Cultures containing testosterone expressed both normal DNA synthesis in MLR and cytotoxic activity in the CML test. These findings suggest a selective pattern of immunosuppression by sex hormones which may be important in preventing graft rejection or graft-versus-host interactions which may arise as a consequence of fetal engraftment during pregnancy.
已对几种甾体激素对近交系小鼠淋巴细胞功能的作用进行了研究。使用混合淋巴细胞反应（MLR）和细胞介导的淋巴细胞溶解（CML）评估体外同种异体细胞相互作用的识别、增殖和效应阶段。在含有Balb/c（反应细胞）和C57bl/6（刺激细胞）脾细胞的MLR中，孕酮、皮质醇或雌二醇存在时DNA合成显著减少。在CML中，孕酮和雌二醇（1 - 5微克/毫升）阻断了体外细胞毒性淋巴细胞的生成，而皮质醇培养物受到部分抑制。这些激素均未抑制体外产生的先前致敏效应细胞的细胞毒性活性。含有睾酮的培养物在MLR中表现出正常的DNA合成，在CML试验中表现出细胞毒性活性。这些发现提示了性激素免疫抑制的一种选择性模式，这在预防移植排斥或妊娠期间胎儿植入可能导致的移植物抗宿主相互作用方面可能很重要。
