Sergeev A V
Biull Eksp Biol Med. 1984 Mar;97(3):327-8.
p-Hydroxyphenyl lactic acid (PHA) in a concentration of 5 . 10(-5) M produced a significant inhibition of cell proliferation in response to alloantigens in a one-way mixed lymphocyte culture (MLC) in colonic cancer patients and in blast transformation in response to suboptimal doses of Con A. Multiple administration of ascorbic acid in an optimal concentration to the culture increased the proliferative response of lymphocytes to alloantigens and Con A. PHA and ascorbic acid did not exhibit any immunomodulating action during the use of healthy donors' lymphocytes or lymphocytes from colonic cancer patients, transformed with optimal mitogen doses. PHA did not affect the production of cytotoxic T lymphocytes in the MLC of the spleens of allogeneic mice but inhibited lymphocyte proliferation in response to alloantigens in the MLC of the spleens obtained from B6 and vitamin A deficient animals.
浓度为5×10⁻⁵ M的对羟基苯乳酸（PHA）在单向混合淋巴细胞培养（MLC）中，对结肠癌患者的同种异体抗原刺激的细胞增殖产生了显著抑制作用，并且对次优剂量的刀豆蛋白A（Con A）刺激的母细胞转化也有抑制作用。向培养物中多次添加最佳浓度的抗坏血酸，可增强淋巴细胞对同种异体抗原和Con A的增殖反应。在使用健康供体淋巴细胞或用最佳有丝分裂原剂量转化的结肠癌患者淋巴细胞时，PHA和抗坏血酸未表现出任何免疫调节作用。PHA不影响同种异体小鼠脾脏MLC中细胞毒性T淋巴细胞的产生，但抑制了从B6和维生素A缺乏动物获得的脾脏MLC中淋巴细胞对同种异体抗原的增殖反应。
