人类细胞中的一种DNA重组活性。
A DNA-recombinogenic activity in human cells.
作者信息
Kenne K, Ljungquist S
出版信息
Nucleic Acids Res. 1984 Apr 11;12(7):3057-68. doi: 10.1093/nar/12.7.3057.
Abstract
A DNA recombining protein has been partly purified from cell lines derived from patients suffering from the hereditary disease, Bloom's syndrome. The protein induces the formation of displacement loops in phi X174 RFI DNA molecules after the addition of single-stranded DNA fragments. A filter binding method and electron microscopy were used to determine the reaction. The recombinogenic protein is dependent on divalent cations and ATP for activity.
摘要
一种DNA重组蛋白已从患有遗传性疾病布卢姆综合征患者的细胞系中部分纯化出来。在加入单链DNA片段后，该蛋白可诱导φX174 RFI DNA分子中置换环的形成。采用滤膜结合法和电子显微镜来确定该反应。这种重组蛋白的活性依赖于二价阳离子和ATP。
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