Higgins D A, Gatrill A J
Int Arch Allergy Appl Immunol. 1984;75(3):219-26. doi: 10.1159/000233619.
The primary and secondary antibody responses of rabbits and badgers were compared after intravenous inoculation of inactivated influenza A virus, sheep erythrocytes (SRBCs), bovine serum albumin (BSA) or bacteriophage psi X174. BSA was also given as a primary injection by the intramuscular route in solution or in Freund's incomplete or complete adjuvant, followed by an intravenous secondary inoculation without adjuvant. Antibody responses were monitored by: haemagglutination inhibition and neutralization tests for influenza virus; direct and antiglobulin haemagglutination tests for SRBCs; indirect haemagglutination test and the Farr method for antigen-binding capacity (ABC) for BSA; neutralization of psi X174. Rabbits gave good responses to all antigens, but the response of badgers was generally poor. After intravenous administration, badgers gave a good response only to psi X174, but even then they produced less antibody than rabbits receiving 100 times less antigen; the immune elimination of phage was more rapid and antibody appeared about 48 h earlier in rabbits than in badgers. Intramuscular administration of BSA and the use of adjuvants improved the badgers' response, with greatest improvement in ABC. These results indicate that badgers display relatively poor immune responses to a variety of antigens.
在静脉注射灭活甲型流感病毒、绵羊红细胞（SRBC）、牛血清白蛋白（BSA）或噬菌体ψX174后，比较了兔和獾的初次和二次抗体反应。BSA也通过肌肉注射途径以溶液形式或在弗氏不完全或完全佐剂中进行初次注射，随后在无佐剂的情况下进行静脉二次接种。通过以下方法监测抗体反应：流感病毒的血凝抑制和中和试验；SRBC的直接和抗球蛋白血凝试验；BSA的间接血凝试验和Farr法测定抗原结合能力（ABC）；ψX174的中和试验。兔对所有抗原均有良好反应，但獾的反应通常较差。静脉给药后，獾仅对ψX174有良好反应，但即便如此，它们产生的抗体仍比接受抗原量少100倍的兔少；噬菌体的免疫清除在兔中更快，抗体出现时间比獾早约48小时。肌肉注射BSA和使用佐剂可改善獾的反应，其中ABC的改善最为显著。这些结果表明，獾对多种抗原的免疫反应相对较差。
