细菌视紫红质光循环中反向光反应的电响应。
Electric response of a back photoreaction in the bacteriorhodopsin photocycle.
作者信息
Ormos P, Dancsházy Z, Keszthelyi L
出版信息
Biophys J. 1980 Aug;31(2):207-13. doi: 10.1016/S0006-3495(80)85051-X.
Abstract
The electric response of a back photoreaction in the bacteriorhodopsin photocycle was investigated. The proton pumping activity of green flash excited bacteriorhodopsin stops if the M412 form is illuminated by blue light (Karvaly and Dancsházy, 1977). In the present work a fast negative displacement current signal was measured in an oriented membrane suspension system, indicative of back movement of protons from M412 to BR570. Quantitative evaluation of the data shows that there are at least two steps in the back reaction, with different rate constants. The temperature dependence of the rate constants show simple linear Arrhenius behavior between 5 degree and 40 degree C. The rate constants were slower by a factor of 1.8 in D2O suspension. The relevance of the protein electric response signals (PERS) observed in this paper to the early receptor potential is discussed.
摘要
研究了细菌视紫红质光循环中反向光反应的电响应。如果用蓝光照射M412形式，绿色闪光激发的细菌视紫红质的质子泵浦活性就会停止（卡尔瓦利和丹奇哈齐，1977年）。在本研究中，在定向膜悬浮系统中测量到一个快速的负位移电流信号，表明质子从M412反向移动到BR570。对数据的定量评估表明，反向反应至少有两个步骤，速率常数不同。速率常数的温度依赖性在5摄氏度至40摄氏度之间呈现简单的线性阿伦尼乌斯行为。在重水悬浮液中，速率常数慢了1.8倍。本文讨论了观察到的蛋白质电响应信号（PERS）与早期感受器电位的相关性。
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本文引用的文献
1
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Science. 1967 Mar 3;155(3766):1128-31. doi: 10.1126/science.155.3766.1128.
2
Electrical evidence for the field indicating absorption change in bioenergetic membranes.表明生物能量膜中吸收变化的电场的电学证据。
FEBS Lett. 1973 Dec 1;37(2):307-10. doi: 10.1016/0014-5793(73)80484-3.
3
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Proc Natl Acad Sci U S A. 1974 Nov;71(11):4462-6. doi: 10.1073/pnas.71.11.4462.
4
Bacteriorhodopsin: a molecular photoelectric regulator. Quenching of photovoltaic effect of bimolecular lipid membranes containing bacteriorhodopsin by blue light.
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5
Kinetics of the blue light-induced inhibition of photoelectric activity of bacteriorhodopsin.
FEBS Lett. 1978 Dec 1;96(1):59-63. doi: 10.1016/0014-5793(78)81062-x.
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7
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