Astrin S M, Crittenden L B, Buss E G
J Virol. 1980 Jan;33(1):250-5. doi: 10.1128/JVI.33.1.250-255.1980.
ev 2 is one of seven recently described genetic loci of chickens which contain structural genes for endogenous virus. ev 2 is present exclusively in line 72 chickens, an inbred strain of white Leghorns which is homozygous for the capacity to produce Rous-associated virus type 0 (RAV-0), a subgroup E virus. This phenotype is known as V+ and has been assigned a genetic allele designated V-E7. The segregation of ev 2 was followed in a genetic cross in which the V-E7+ phenotype was also segregating. The progeny of the cross were analyzed for endogenous viral loci by cleavage of embryo DNA with restriction endonuclease SstI, electrophoretic separation of the resulting fragments, and identification of bands containing viral sequences by hybridization of the DNA to radiolabeled viral RNA. Four endogenous viral loci, ev 1, ev 2, ev 4, and ev 5, were identified in the progeny of the cross. One of the progeny contained no detectable endogenous viral sequences. ev 1, ev 4, and ev 5 were present in progeny of both the V-E7+ and V-E7- phenotypes. ev 2 was present exclusively in progeny of the V-E7+ phenotype, and all V-E7+ progeny contained ev 2. In addition, one of the V-E7+ progeny contained only ev 2. FRom these data, we conclude that ev 2 codes for RAV-0 virus produced by the cells of line 72 chickens.
ev 2是鸡最近被描述的七个遗传位点之一，这些位点包含内源性病毒的结构基因。ev 2仅存在于72系鸡中，72系鸡是白来航鸡的近交系，对于产生0型劳斯相关病毒（RAV - 0，一种E亚群病毒）的能力是纯合的。这种表型被称为V +，并被赋予了一个指定为V - E7的遗传等位基因。在一个遗传杂交中追踪ev 2的分离情况，其中V - E7 +表型也在分离。通过用限制性内切酶SstI切割胚胎DNA、对所得片段进行电泳分离以及通过将DNA与放射性标记的病毒RNA杂交来鉴定含有病毒序列的条带，对杂交后代的内源性病毒位点进行了分析。在杂交后代中鉴定出了四个内源性病毒位点，即ev 1、ev 2、ev 4和ev 5。其中一个后代没有可检测到的内源性病毒序列。ev 1、ev 4和ev 5存在于V - E7 +和V - E7 -表型的后代中。ev 2仅存在于V - E7 +表型的后代中，并且所有V - E7 +后代都含有ev 2。此外，一个V - E7 +后代仅含有ev 2。根据这些数据，我们得出结论，ev 2编码72系鸡细胞产生的RAV - 0病毒。
