Crittenden L B, Astrin S M, Smith E J
Virology. 1983 Sep;129(2):514-6. doi: 10.1016/0042-6822(83)90192-7.
F1 males produced by crossing Regional Poultry Research Laboratory line 15I4 that carries endogenous viral (ev) genes 1, 6, and 10 and a partially defined gene, ev 11, with line 15B that carries ev 1 and 7 were backcrossed to line 15B females. The DNA of 72 progeny embryos was digested by BamHI and Kpn and hybridization of resulting fragments with 32P-labeled viral RNA showed that ev 6, 10, and 11 were autosomal genes and not closely linked, and confirmed that ev 7 was sex linked. ev 10 and 11 were consistently associated with complete virus production, confirming that the newly defined ev 11 codes for a complete endogenous retrovirus.
将携带内源性病毒（ev）基因1、6和10以及一个部分确定的基因ev 11的区域家禽研究实验室品系15I4与携带ev 1和7的品系15B杂交产生的F1雄性与品系15B雌性回交。用BamHI和Kpn消化72个后代胚胎的DNA，并用32P标记的病毒RNA对所得片段进行杂交，结果表明ev 6、10和11是常染色体基因且没有紧密连锁，并证实ev 7是性连锁的。ev 10和11始终与完整病毒的产生相关，证实新确定的ev 11编码一种完整的内源性逆转录病毒。
