立克次氏体与巨噬细胞相互作用的体外研究：紫外线对贝氏柯克斯体灭活及巨噬细胞酶的影响
In vitro studies of interaction of rickettsia and macrophages: effect of ultraviolet light on Coxiella burnetii inactivation and macrophage enzymes.
作者信息
Little J S, Kishimoto R A, Canonico P G
出版信息
Infect Immun. 1980 Mar;27(3):837-41. doi: 10.1128/iai.27.3.837-841.1980.
The inactivation of Coxiella burnetii in suspension or in cultures of guinea pig peritoneal macrophages by ultraviolet (UV) light was studied. The effect of UV treatment on the activity of macrophage organelle marker enzymes and their subsequent equilibration in linear sucrose gradients was also determined. It was shown that UV treatment of 600 mu W/cm2 for 15 s at a distance of 10 cm inactivated C. burnetti, either in suspension (10(8) organisms per ml) or within guinea pig peritoneal macrophages. Similar UV treatment had little effect on the activity or equilibration of macrophage organelle marker enzymes in linear sucrose gradients. However, longer exposure caused considerable inactivation of these enzymes.
研究了紫外线（UV）对悬浮状态或豚鼠腹膜巨噬细胞培养物中伯氏考克斯体的灭活作用。还测定了紫外线处理对巨噬细胞细胞器标记酶活性及其随后在线性蔗糖梯度中的平衡的影响。结果表明，在距离10 cm处用600 μW/cm²的紫外线处理15 s，可使悬浮状态（每毫升10⁸个菌体）或豚鼠腹膜巨噬细胞内的伯氏考克斯体失活。类似的紫外线处理对线性蔗糖梯度中巨噬细胞细胞器标记酶的活性或平衡几乎没有影响。然而，较长时间的照射会导致这些酶大量失活。
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