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嗜热四膜虫大核中rRNA基因的分离与定位

Isolation and mapping of the rRNA genes in the macronucleus of Oxytricha fallax.

作者信息

Spear B B

出版信息

Chromosoma. 1980;77(2):193-202. doi: 10.1007/BF00329544.

DOI:10.1007/BF00329544
PMID:6248307
Abstract

The DNA in the macronucleus of the protozoan Oxytricha, unlike like that of typical eukaryotes, exists as short, gene-sized molecules. Within the macronucleus the rRNA genes are contained in molecules 7,380 nucleotide pairs in length. This rDNA has been substantially purified by selective denaturation of non-ribosomal DNA followed by S1 nuclease digestion. Results from restriction nuclease digestion and rRNA:DNA hybridization who that the rDNA is a linear, non-palindromic molecule which contains one gene each for the 19s and 25s rRNAs. A total of less than 600 base pairs of DNA lies between the 19s and 25s genes or at the 3' end of the 25s gene. The non-coding portion of the ribosomal DNA is almost entirely limited to an approximately 1,400 base pair region at the 5' end of the molecule.

摘要

与典型真核生物不同，原生动物嗜热四膜虫大核中的DNA以短的、基因大小的分子形式存在。在大核中，rRNA基因包含在长度为7380个核苷酸对的分子中。通过非核糖体DNA的选择性变性，然后用S1核酸酶消化，这种rDNA已被大量纯化。限制性核酸酶消化和rRNA:DNA杂交的结果表明，rDNA是一种线性、非回文分子，它包含一个19s和25s rRNA的基因。19s和25s基因之间或25s基因的3'端共有不到600个碱基对的DNA。核糖体DNA的非编码部分几乎完全局限于分子5'端大约1400个碱基对的区域。

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Arrangement of coding and non-coding sequences in the DNA molecules coding for rRNAs in Oxytricha sp. DNA of ciliated protozoa. VII.嗜热四膜虫属纤毛原生动物的DNA中编码rRNA的DNA分子的编码和非编码序列的排列。VII.
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