Weststrate M W, Geelen J L, van der Noordaa J
J Gen Virol. 1980 Jul;49(1):1-21. doi: 10.1099/0022-1317-49-1-1.
It is proposed that the genome of human cytomegalovirus (HCMV) consists of two unique sequences, L and S, bounded by two sets of redundant sequences (P. Sheldrick et al. unpublished data). In this arrangement the terminal sequences (TR1 and TR8) are repeated in an intenal inverted form (IR1 and IR8) and delimit L and S. After restriction endonuclease cleavage of the DNA, four o.5 M and four 0.25 M fragments are found, indicating that HCMV DNA preparations consist of four equimolar populations differing only in the relative orientation of the L and S components. Cleavage of the CMV DNA with the restriction endonucleases BglII, HindIII and XbaI results in 32, 27 and 21 fragments, respectively. The arrangement of these fragments has been determined using molecular hybridization techniques, identification of terminal fragments and the identification of linkage groups by double-digestion. In this report the physical maps for the restriction endonucleases BglII, HindIII and XbaI are presented.
有人提出，人类巨细胞病毒（HCMV）的基因组由两个独特序列L和S组成，其两侧是两组重复序列（P. Sheldrick等人未发表的数据）。在这种排列中，末端序列（TR1和TR8）以内部反向形式（IR1和IR8）重复，并界定L和S。用限制性内切酶切割DNA后，发现四个0.5M片段和四个0.25M片段，这表明HCMV DNA制剂由四个等摩尔群体组成，它们仅在L和S成分的相对取向上有所不同。用限制性内切酶BglII、HindIII和XbaI切割CMV DNA分别产生32、27和21个片段。已使用分子杂交技术、末端片段鉴定和通过双酶切鉴定连锁群来确定这些片段的排列。在本报告中，给出了限制性内切酶BglII、HindIII和XbaI的物理图谱。
