Field H J, Darby G, Wildy P
J Gen Virol. 1980 Jul;49(1):115-24. doi: 10.1099/0022-1317-49-1-115.
Mutants of HSV which are resistant to acyclovir (acycloguanosine) have been isolated following serial passages of several herpes simplex virus (HSV) strains in the presence of the drug. The majority of the mutants isolated are defective in induction of thymidine kinase (TK) and this is consistent with the observation that independently isolated TK- viruses are naturally resistant to ACV. One mutant is described (SC16 R9C2) which is resistant in biochemically transformed cells which express HSV TK. This suggests that its resistance resides at a level other than TK. It is also resistant to phosphonoacetic acid, suggesting that the DNA polymerase locus may be involved. A further mutant is described [Cl (101) P2C5] which induces normal levels of TK, although the nature of resistance of this virus is not yet elucidated.
在几种单纯疱疹病毒（HSV）毒株于阿昔洛韦（无环鸟苷）存在的情况下连续传代后，已分离出对阿昔洛韦耐药的HSV突变体。分离出的大多数突变体在胸苷激酶（TK）诱导方面存在缺陷，这与独立分离的TK-病毒天然对阿昔洛韦耐药的观察结果一致。描述了一种突变体（SC16 R9C2），其在表达HSV TK的生化转化细胞中具有耐药性。这表明其耐药性存在于TK以外的水平。它对膦甲酸也具有耐药性，提示DNA聚合酶位点可能涉及其中。描述了另一种突变体[Cl(101) P2C5]，其诱导正常水平的TK，尽管该病毒耐药的本质尚未阐明。
