Rao M S, Reddy J K
J Natl Cancer Inst. 1977 Feb;58(2):387-92. doi: 10.1093/jnci/58.2.387.
The effect of chronic administration of 2,2'-dihydroxy-di-n-propylnitrosamine (DHPN) was studied in randombred guinea pigs. DHPN, dissolved in olive oil, was injected sc into 40 animals at a dose of 250 mg/kg body weight/week for 30 weeks, and the animals were observed until their death or termination of the experiment at the end of 40 weeks. Of the 32 guinea pigs that survived more than 20 weeks of DHPN treatment, 23 developed angiosarcoma of the liver between 22 and 40 weeks. Metastases to lungs, spleen, and peripancreatic lymph nodes were observed in 8 animals. Other tumors included hepatocellular carcinoma (1 animal), cholangiocarcinoma (1 animal), chronic myeloid leukemia (1 animal), acinar cell adenoma of pancreas (1 animal), and acinar cell carcinoma of pancreas (1 animal). In addition, megalocytic change of hepatic cells with intranuclear inclusions, pelliosis hepatis, and cholangiomatous lesions were also encountered frequently in the livers.
在随机繁殖的豚鼠中研究了长期给予2,2'-二羟基-二正丙基亚硝胺（DHPN）的效果。将溶解于橄榄油中的DHPN以250mg/kg体重/周的剂量皮下注射给40只动物，持续30周，并观察这些动物直至其死亡或在40周结束时终止实验。在接受DHPN治疗超过20周的32只豚鼠中，23只在22至40周之间发生了肝血管肉瘤。在8只动物中观察到转移至肺、脾和胰周淋巴结。其他肿瘤包括肝细胞癌（1只动物）、胆管癌（1只动物）、慢性粒细胞白血病（1只动物）、胰腺腺泡细胞腺瘤（1只动物）和胰腺腺泡细胞癌（1只动物）。此外，肝脏中还经常出现伴有核内包涵体的肝细胞巨细胞改变、肝紫癜和胆管瘤样病变。
