Steinmetz M, Altenburger W, Zachau H G
Nucleic Acids Res. 1980 Apr 25;8(8):1709-20. doi: 10.1093/nar/8.8.1709.
A 5.3 kb EcoRI fragment (T3, abbreviations in ref. 2) has been cloned from DNA of a kappa light chain producing mouse myeloma. The fragment hybridizes to the k' flanking sequences of the J1 gene segment but not to C gene sequences of kappa light chain DNA. Restriction nuclease mapping and partial nucleotide sequencing showed that the fragment consists of sequences from the 5' side of the J1 and form the 3' side of a V gene segment, which apparently had been linked in a genomic rearrangement process. These rearranged flanking sequences are not the flanking sequences of the V and J gene segments which had been joined to form the two kappa light chain genes of the myeloma. Fragments with the hybridization properties of T3 have been found also in two other kappa and one lambda chain producing myelomas. The linking of flanking sequences in the myeloma genome is discussed with respect to the mechanism of recombination between V and J gene segments.
从产生κ轻链的小鼠骨髓瘤DNA中克隆出了一个5.3 kb的EcoRI片段（T3，参考文献2中的缩写）。该片段与J1基因片段的κ'侧翼序列杂交，但不与κ轻链DNA的C基因序列杂交。限制性核酸酶图谱分析和部分核苷酸测序表明，该片段由J1 5'端的序列和一个V基因片段3'端的序列组成，这些序列显然在基因组重排过程中连接在一起。这些重排的侧翼序列不是与骨髓瘤的两个κ轻链基因形成连接的V和J基因片段的侧翼序列。在另外两种产生κ轻链的骨髓瘤和一种产生λ轻链的骨髓瘤中也发现了具有T3杂交特性的片段。本文就V和J基因片段之间的重组机制讨论了骨髓瘤基因组中侧翼序列的连接情况。
