小核糖核酸病毒VPg序列包含在复制酶前体中。
Picornaviral VPg sequences are contained in the replicase precursor.
作者信息
Pallansch M A, Kew O M, Palmenberg A C, Golini F, Wimmer E, Rueckert R R
出版信息
J Virol. 1980 Aug;35(2):414-9. doi: 10.1128/JVI.35.2.414-419.1980.
Abstract
It has previously been shown that the RNA replicase of encephalomyocarditis virus contains two virus-coded proteins, D and E, which are produced in two successive proteolytic steps: (i) C leads to D + ?; and (ii) D leads to p22 + E. It is here shown (i) that virus protein H (molecular weight, 12,000) is the previously unidentified product of the first step and (ii) that VPg, a protein linked covalently to the virion RNA, yields two tryptic peptides found in protein C but not in protein D. The results suggest that VPg is derived by cleavage of protein C and that protein H may be intermediate. Preliminary experiments with VPg sequences in polioviral noncapsid protein 1b, the counterpart of encephalomyocarditis viral protein C, were inconclusive.
摘要
先前的研究表明，脑心肌炎病毒的RNA复制酶包含两种病毒编码蛋白，D和E，它们通过两个连续的蛋白水解步骤产生：（i）C产生D +？； （ii）D产生p22 + E。本文表明：（i）病毒蛋白H（分子量12,000）是第一步中先前未鉴定的产物； （ii）与病毒体RNA共价连接的蛋白VPg产生了在蛋白C中发现但在蛋白D中未发现的两种胰蛋白酶肽。结果表明，VPg是由蛋白C裂解产生的，蛋白H可能是中间产物。对脊髓灰质炎病毒非衣壳蛋白1b（脑心肌炎病毒蛋白C的对应物）中的VPg序列进行的初步实验尚无定论。
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