Grubman M J, Robertson B H, Morgan D O, Moore D M, Dowbenko D
J Virol. 1984 May;50(2):579-86. doi: 10.1128/JVI.50.2.579-586.1984.
Pulse-chase labeling of foot-and-mouth disease virus-infected bovine kidney cells revealed stable and unstable viral-specific polypeptides. To identify precursor-product relationships among these polypeptides, antisera against a number of structural and nonstructural viral-specific polypeptides were used. Cell-free translations programmed with foot-and-mouth disease virion RNA or foot-and-mouth disease virus-infected bovine kidney cell lysates, which were shown to contain almost identical polypeptides, were immunoprecipitated with the various antisera. To further establish identity, some proteins were compared by partial protease digestion. Evidence for a membrane association of the polypeptides coded for by the middle genome region is also presented. A biochemical map of the foot-and-mouth disease virus genome was established from the above information.
对感染口蹄疫病毒的牛肾细胞进行脉冲追踪标记，揭示了稳定和不稳定的病毒特异性多肽。为了确定这些多肽之间的前体-产物关系，使用了针对多种结构和非结构病毒特异性多肽的抗血清。用口蹄疫病毒粒子RNA或感染口蹄疫病毒的牛肾细胞裂解物进行无细胞翻译，结果显示它们含有几乎相同的多肽，然后用各种抗血清进行免疫沉淀。为了进一步确定同一性，通过部分蛋白酶消化对一些蛋白质进行了比较。还提供了中基因组区域编码的多肽与膜相关的证据。根据上述信息建立了口蹄疫病毒基因组的生化图谱。
