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小鼠免疫球蛋白Cγ3和Cγ1基因侧翼的克隆胚胎DNA序列。

Cloned embryonic DNA sequences flanking the mouse immunoglobulin C gamma 3 and C gamma 1 genes.

作者信息

Adams J M, Webb E, Gerondakis S, Cory S

出版信息

Nucleic Acids Res. 1980 Dec 20;8(24):6019-32. doi: 10.1093/nar/8.24.6019.

DOI:10.1093/nar/8.24.6019
PMID:6258159
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328069/
Abstract

To investigate the DNA surrounding genes for immunoglobulin heavy chain constant (CH) regions, we have isolated two clones bearing a C gamma 3 gene and two bearing a C gamma 1 gene from a library of mouse embryo DNA fragments. The C gamma 3 clones span 8.6 kilobase pairs (kb) on the 5' side of the gene and 6.7 kb on its 3' side, while the C gamma 1 clones together span 13 kb of 5' flanking sequence and 2.5 kb of 3' flanking sequence. Restriction mapping of the C gamma 3 gene indicates that intervening sequences divide the gene into segments of domain size, as in other CH genes. Hybridization of clone fragments to restriction digests of mouse DNA indicates that both the C gamma 1 and C gamma 3 genes probably occur as single copies in the genome. Moreover, the entire cloned sequences on the 5' side of both genes appear to be unique in the genome, indicating that no large common sequences flank CH genes. Restriction data suggest that the C gamma 3 gene is 37-40 kb 5' to the C gamma 1 gene.

摘要

为了研究免疫球蛋白重链恒定（CH）区基因周围的DNA，我们从小鼠胚胎DNA片段文库中分离出两个携带Cγ3基因的克隆和两个携带Cγ1基因的克隆。Cγ3克隆在基因的5'端跨度为8.6千碱基对（kb），在其3'端跨度为6.7 kb，而Cγ1克隆一起跨越13 kb的5'侧翼序列和2.5 kb的3'侧翼序列。Cγ3基因的限制性图谱表明，间隔序列将该基因分成结构域大小的片段，就像其他CH基因一样。克隆片段与小鼠DNA限制性消化产物的杂交表明，Cγ1和Cγ3基因在基因组中可能都是单拷贝存在。此外，两个基因5'端的整个克隆序列在基因组中似乎都是独特的，这表明CH基因两侧没有大的共同序列。限制性数据表明，Cγ3基因在Cγ1基因的5'端37 - 40 kb处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/08adf93f4c74/nar00441-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/c5a817ef0f34/nar00441-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/b03b4920068f/nar00441-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/1cbe7c2022fc/nar00441-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/36dbad89ff87/nar00441-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/c98ba4dc12df/nar00441-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/08adf93f4c74/nar00441-0105-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/c5a817ef0f34/nar00441-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/b03b4920068f/nar00441-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/1cbe7c2022fc/nar00441-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/36dbad89ff87/nar00441-0104-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/c98ba4dc12df/nar00441-0104-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61cd/328069/08adf93f4c74/nar00441-0105-a.jpg

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本文引用的文献

1
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Proc Natl Acad Sci U S A. 1980 Aug;77(8):4943-7. doi: 10.1073/pnas.77.8.4943.
2
Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.产生完整的免疫球蛋白重链基因需要两种类型的体细胞重组。
Nature. 1980 Aug 14;286(5774):676-83. doi: 10.1038/286676a0.
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An immunoglobulin deletion mutant with implications for the heavy-chain switch and RNA splicing.
在小鼠淋巴瘤中频繁重排的转录活性DNA区域。
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On immunoglobulin heavy chain gene switching: two gamma 2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed.关于免疫球蛋白重链基因转换：在MPC - 11细胞中，两个γ2b基因通过转换序列进行重排，但只有一个表达。
Nucleic Acids Res. 1982 Jan 22;10(2):611-30. doi: 10.1093/nar/10.2.611.
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Transcriptional regulation of the mu-delta heavy chain locus in normal murine B lymphocytes.正常小鼠B淋巴细胞中μ-δ重链基因座的转录调控。
J Exp Med. 1984 Aug 1;160(2):564-83. doi: 10.1084/jem.160.2.564.
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Structural analysis of the murine IgG3 constant region gene.小鼠IgG3恒定区基因的结构分析
EMBO J. 1984 Sep;3(9):2041-6. doi: 10.1002/j.1460-2075.1984.tb02089.x.
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Unique sequences are interspersed among tandemly repeated elements in the murine gamma 1 switch segment.在小鼠γ1转换区段中，独特序列散布于串联重复元件之间。
Nucleic Acids Res. 1985 Jan 11;13(1):225-37. doi: 10.1093/nar/13.1.225.
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Structure and expression of mouse germline immunoglobulin gamma 3 heavy chain transcripts induced by the mitogen lipopolysaccharide.由促有丝分裂原脂多糖诱导的小鼠种系免疫球蛋白γ3重链转录本的结构与表达
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Rearrangement of immunoglobulin heavy chain genes during B-lymphocyte development as revealed by studies of mouse plasmacytoma cells.通过对小鼠浆细胞瘤细胞的研究揭示B淋巴细胞发育过程中免疫球蛋白重链基因的重排。
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