Charles S A, Halliwell B
Biochem J. 1980 Jun 15;188(3):775-9. doi: 10.1042/bj1880775.
Thiol-treated spinach (Spinacia oleracea) chloroplast fructose bisphosphatase is powerfully inhibited by Ca2+ non-competitively with respect to its substrate, fructose 1,6-bisphosphate. 500 microM-Ca2+ causes virtually complete inhibition and the Ki is 40 microM. Severe inhibition of sedoheptulose bisphosphatase is also caused by Ca2+. A role for Ca2+ in regulation of the Calvin cycle in spinach chloroplasts is proposed.
经硫醇处理的菠菜(Spinacia oleracea)叶绿体果糖二磷酸酶,相对于其底物1,6-二磷酸果糖,会受到Ca2+的强烈非竞争性抑制。500微摩尔/升的Ca2+几乎能导致完全抑制,其抑制常数(Ki)为40微摩尔/升。Ca2+也会严重抑制景天庚酮糖二磷酸酶。本文提出了Ca2+在菠菜叶绿体卡尔文循环调控中的作用。