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单纯疱疹病毒1型和2型多肽经电泳分离并转移至重氮苄氧基甲基纸上后的免疫反应性。

Immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.

作者信息

Norrild B, Pedersen B, Roizman B

出版信息

Infect Immun. 1981 Feb;31(2):660-7. doi: 10.1128/iai.31.2.660-667.1981.

DOI:10.1128/iai.31.2.660-667.1981
PMID:6260673
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC351360/
Abstract

In this paper we report that viral polypeptides from herpes simplex virus 1 (HSV-1) and 2 (HSV-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. The polyvalent hyperimmune sera against HSV-1 reacted with 17 HSV-1 polypeptide bands and 8 HSV-2 polypeptide bands. Concordantly, polyvalent sera against HSV-2 reacted with at least 16 HSV-2 polypeptide bands and 8 HSV-1 polypeptide bands. The antisera prepared against the specific antigens reacted with a smaller number of polypeptide bands. Preimmune sera and immune sera did not react with electrophoretically separated polypeptides from infected and uninfected cells, respectively. The immune localization of separated antigens test provides a powerful technique for identification of immunogenic viral polypeptides, especially those which are normally insoluble and therefore unavailable for immunological reactivity in immune precipitation tests.

摘要

在本文中，我们报道了从单纯疱疹病毒1型（HSV-1）和2型（HSV-2）感染细胞中提取的病毒多肽，经十二烷基硫酸钠-聚丙烯酰胺-琼脂糖凝胶电泳分离后转移至重氮苄氧基甲基纸上，可与兔超免疫血清发生反应，包括多价血清以及针对特定抗原制备的血清。针对HSV-1的多价超免疫血清与17条HSV-1多肽带和8条HSV-2多肽带发生反应。同样，针对HSV-2的多价血清与至少16条HSV-2多肽带和8条HSV-1多肽带发生反应。针对特定抗原制备的抗血清与较少数量的多肽带发生反应。免疫前血清和免疫血清分别不与来自感染和未感染细胞的经电泳分离的多肽发生反应。分离抗原的免疫定位试验为鉴定免疫原性病毒多肽提供了一种强大的技术，尤其是那些通常不溶性因而在免疫沉淀试验中无法进行免疫反应的多肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/fe1ce236b164/iai00166-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/aaa6c8c4f24e/iai00166-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/12be8e9410ca/iai00166-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/1e562842097a/iai00166-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/ae0172f1dc2f/iai00166-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/fe1ce236b164/iai00166-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/aaa6c8c4f24e/iai00166-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/12be8e9410ca/iai00166-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/1e562842097a/iai00166-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/ae0172f1dc2f/iai00166-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4099/351360/fe1ce236b164/iai00166-0158-a.jpg

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J Virol. 1983 Apr;46(1):103-12. doi: 10.1128/JVI.46.1.103-112.1983.
2
Secretory IgA specific for herpes simplex virus in lacrimal fluid from patients with herpes keratitis--a possible diagnostic parameter.疱疹性角膜炎患者泪液中针对单纯疱疹病毒的分泌型 IgA——一种可能的诊断参数。
Br J Ophthalmol. 1982 Oct;66(10):648-53. doi: 10.1136/bjo.66.10.648.
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本文引用的文献

1
Type-common and type-specific monoclonal antibody to herpes simplex virus type 1.针对1型单纯疱疹病毒的通用型和特异型单克隆抗体。
Infect Immun. 1980 Aug;29(2):724-32. doi: 10.1128/iai.29.2.724-732.1980.
2
Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA.单纯疱疹病毒磷蛋白。I. 磷酸基团在一些病毒多肽上循环进出，并且能够改变它们对DNA的亲和力。
J Virol. 1980 Jan;33(1):167-82. doi: 10.1128/JVI.33.1.167-182.1980.
3
Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates.
Effect of tunicamycin on the synthesis of herpes simplex virus type 1 glycoproteins and their expression on the cell surface.
衣霉素对单纯疱疹病毒1型糖蛋白合成及其在细胞表面表达的影响。
J Virol. 1982 Aug;43(2):395-402. doi: 10.1128/JVI.43.2.395-402.1982.
通过免疫沉淀物的聚丙烯酰胺凝胶电泳研究单纯疱疹病毒特异性多肽。
J Gen Virol. 1974 Feb;22(2):171-85. doi: 10.1099/0022-1317-22-2-171.
4
Studies on the relatedness of herpesviruses through DNA-DNA hybridization.通过DNA-DNA杂交对疱疹病毒相关性的研究。
Virology. 1972 Jul;49(1):95-101. doi: 10.1016/s0042-6822(72)80010-2.
5
Genetic relatedness of type 1 and type 2 herpes simplex viruses.1型和2型单纯疱疹病毒的遗传相关性。
J Virol. 1972 May;9(5):738-45. doi: 10.1128/JVI.9.5.738-745.1972.
6
Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2.单纯疱疹病毒1型和2型脱氧核糖核酸的大小、组成及结构
J Virol. 1971 Aug;8(2):125-32. doi: 10.1128/JVI.8.2.125-132.1971.
7
Antigens of Herpes simplex virus type 1 and 2-immunodiffusion and inhibition passive hemagglutination studies.1型和2型单纯疱疹病毒抗原——免疫扩散及抑制被动血凝试验研究
Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):471-87.
8
Herpesvirus antigens on cell membranes detected by centrifugation of membrane-antibody complexes.通过膜抗体复合物离心检测细胞膜上的疱疹病毒抗原。
Science. 1971 Jan 22;171(3968):298-300. doi: 10.1126/science.171.3968.298.
9
Density difference of DNA of human herpes simplex viruses, types I and II.人类单纯疱疹病毒I型和II型DNA的密度差异
Virology. 1968 Jul;35(3):473-5. doi: 10.1016/0042-6822(68)90225-0.
10
Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus.1型单纯疱疹病毒感染细胞以及该病毒裸露颗粒和包膜颗粒表面的型特异性和型共同抗原。
J Gen Virol. 1974 Feb;22(2):159-69. doi: 10.1099/0022-1317-22-2-159.