Forghani B, Schmidt N J
J Med Virol. 1980;6(2):119-27. doi: 10.1002/jmv.1890060204.
An enzyme immunoassay utilizing a fluorogenic substrate (EIFA) was employed to compare humoral antibody responses to purified virion and dense body antigens in human cytomegalovirus (CMV) infections. Results of these antibody assays were also compared to those obtained by plaque reduction neutralization and complement fixation (CF). In adults with CMV infections diagnosed by a significant rise in CF antibody titer, there was also a vigorous antibody response to the virion and dense body antigens. There was better correlation between antibody levels to the virion and dense body antigens than between antibody levels detected by other combinations of tests. Antibody responses to the virion and dense body antigens were similar, but not identical, suggesting that although the two structures share major antigens, they may also possess unique antigens to which the host can mount independent antibody responses. All individuals with CMV neutralizing antibody also had virion and dense body antibodies, but there was poor correlation between neutralization titers and those detected by EIFA, perhaps reflecting greater strain specificity of the neutralization test. Some individuals with serological diagnoses of Mycoplasma pneumoniae or influenza A infection showed fourfold or greater antibody titer rises to CMV in two or more assays, suggesting reactivation of latent CMV in these infections.
采用一种利用荧光底物的酶免疫测定法（EIFA）来比较人巨细胞病毒（CMV）感染中针对纯化病毒体和致密体抗原的体液抗体反应。这些抗体测定的结果也与通过蚀斑减少中和试验和补体结合试验（CF）获得的结果进行了比较。在通过CF抗体滴度显著升高诊断为CMV感染的成年人中，对病毒体和致密体抗原也有强烈的抗体反应。与病毒体和致密体抗原的抗体水平之间的相关性，比通过其他检测组合检测到的抗体水平之间的相关性更好。对病毒体和致密体抗原的抗体反应相似，但不完全相同，这表明尽管这两种结构共享主要抗原，但它们也可能拥有宿主能够产生独立抗体反应的独特抗原。所有具有CMV中和抗体的个体也都有病毒体和致密体抗体，但中和滴度与EIFA检测到的滴度之间的相关性较差，这可能反映了中和试验具有更高的菌株特异性。一些血清学诊断为肺炎支原体或甲型流感感染的个体，在两项或更多检测中显示对CMV的抗体滴度升高了四倍或更多，这表明在这些感染中潜伏的CMV被重新激活。
