Grauballe P C, Vestergaard B F, Meyling A, Genner J
J Med Virol. 1981;7(1):29-40. doi: 10.1002/jmv.1890070104.
Detection of human and bovine rotavirus in stools is described using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) with poly-styrene microtest plates as solid phase, immunoglobulin fraction of rabbit antiserum to rotavirus (human) as catching antibody, and the same reagent labelled with horseradish peroxidase as conjugate. The ELISA has been optimized with regard to simplicity, rapidity, sensitivity, and specificity. In a comparative study, stool specimens from 81 infants and children and 92 neonatal calves with diarrhoea were tested for rotavirus by ELISA, electron microscopy (EM), immunoelectro-osmophoresis (IEOP), and fluorescent antibody technique (FA). The relative sensitivity of the different assays for human and bovine rotavirus was: EM 68%, 76%; IEOP 80%, 76%; FA not determined, 85%; and ELISA 86%, 98%, respectively. Less than 1 ng of purified human rotavirus could be detected in ELISA, whereas 100 ng was the minimal amount detected by IEOP. It is concluded that the developed ELISA is a simple, rapid, reliable, and sensitive method for the diagnosis of human and bovine rotavirus infections.
本文描述了一种采用双抗体夹心酶联免疫吸附测定法（ELISA）检测粪便中人和牛轮状病毒的方法。该方法以聚苯乙烯微量试验板为固相，以兔抗轮状病毒（人）血清的免疫球蛋白组分作为捕获抗体，并用辣根过氧化物酶标记的相同试剂作为结合物。该ELISA在简便性、快速性、敏感性和特异性方面均已得到优化。在一项对比研究中，采用ELISA、电子显微镜（EM）、免疫电渗电泳（IEOP）和荧光抗体技术（FA）对81例腹泻婴幼儿和92例腹泻新生小牛的粪便标本进行轮状病毒检测。不同检测方法对人和牛轮状病毒的相对敏感性分别为：EM 68%、76%；IEOP 80%、76%；FA未测定、85%；ELISA 86%、98%。ELISA可检测到少于1 ng的纯化人轮状病毒，而IEOP检测到的最小量为100 ng。结论是，所开发的ELISA是一种用于诊断人和牛轮状病毒感染的简单、快速、可靠且灵敏的方法。
