Grauballe P C, Genner J, Meyling A, Hornsleth A
J Gen Virol. 1977 May;35(2):203-18. doi: 10.1099/0022-1317-35-2-203.
Eighty-seven faecal samples from infants and children suffering from acute gastroenteritis were investigated for the presence of rotavirus by immunoelectroosmophoresis (IEOP) and electron microscopy (EM). Sixty-one % of the samples contained rotavirus antigens when examined by IEOP whereas only 50% were diagnosed as positive EM. However, where it was possible to perform EM within the same day that the sample was received it took 24 h to establish the diagnosis by IEOP. The high sensitivity of the IEOP method was achieved by application of antiserum produced in rabbits to rotavirus immunoprecipitates. The specificity and sensitivity of the diagnostic antiserum produced were tested by application of different immunoelectrophoretic methods.
采用免疫电渗电泳（IEOP）和电子显微镜（EM）对87份患有急性肠胃炎的婴幼儿粪便样本进行轮状病毒检测。通过IEOP检测时，61%的样本含有轮状病毒抗原，而EM诊断的阳性率仅为50%。然而，若在收到样本的同一天进行EM检测，通过IEOP确诊则需要24小时。通过将兔抗血清应用于轮状病毒免疫沉淀物，实现了IEOP方法的高灵敏度。通过应用不同的免疫电泳方法对所制备诊断抗血清的特异性和灵敏度进行了检测。
