直接超速离心后，采用对流免疫电泳、酶免疫测定法及电子显微镜技术检测人轮状病毒。
Human rotavirus detection by counterimmunoelectrophoresis versus enzyme immunoassay and electron microscopy after direct ultracentrifugation.
作者信息
Hammond G W, Ahluwalia G S, Klisko B, Hazelton P R
出版信息
J Clin Microbiol. 1984 Mar;19(3):439-41. doi: 10.1128/jcm.19.3.439-441.1984.
Abstract
The sensitivity of the counterimmunoelectrophoresis test with NCDV, Wa, and SA-11 rotavirus antisera was 60, 60, and 67%, respectively. The counterimmunoelectrophoresis specificity was greater than 99%, but the low sensitivity is a limiting feature of this test as a first-line immunodiagnostic test for rotavirus detection.
摘要
使用NCDV、Wa和SA - 11轮状病毒抗血清进行对流免疫电泳试验的敏感度分别为60%、60%和67%。对流免疫电泳的特异性大于99%，但低敏感度是该试验作为轮状病毒检测一线免疫诊断试验的一个限制因素。
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