Nishiyama Y, Rapp F
J Virol. 1981 Apr;38(1):164-72. doi: 10.1128/JVI.38.1.164-172.1981.
Plaque formation in Vero cells by UV-irradiated herpes simplex virus was enhanced by infection with human cytomegalovirus (HCMV), UV irradiation, or treatment with methylmethanesulfonate. Preinfection of Vero cells with HCMV enhanced reactivation of UV-irradiated herpes simplex virus more significantly than did treatment with UV or methylmethanesulfonate alone. A similar enhancement by HCMV was observed in human embryonic fibroblasts, but not in xeroderma pigmentosum (XP12BE) cells. It was also found that HCMV infection enhanced hydroxyurea-resistant DNA synthesis induced by UV light or methylmethanesulfonate. Alkaline sucrose gradient sedimentation analysis revealed an enhanced rate of synthesis of all size classes of DNA in UV-irradiated HCMV-infected Vero cells. However, HCMV infection did not induce repairable lesions in cellular DNA and did not significantly inhibit host cell DNA synthesis, unlike UV or methylmethanesulfonate. These results indicate that HCMV enhanced DNA repair capacity in the host cells without producing detectable lesions in cellular DNA and without inhibiting DNA synthesis. This repair appeared to be error proof for UV-damaged herpes simplex virus DNA when tested with herpes simplex virus thymidine kinase-negative mutants.
人巨细胞病毒（HCMV）感染、紫外线照射或甲磺酸甲酯处理可增强紫外线照射的单纯疱疹病毒在Vero细胞中的噬斑形成。用HCMV预感染Vero细胞比单独用紫外线或甲磺酸甲酯处理更显著地增强了紫外线照射的单纯疱疹病毒的复活。在人胚成纤维细胞中观察到HCMV有类似的增强作用，但在着色性干皮病（XP12BE）细胞中未观察到。还发现HCMV感染增强了紫外线或甲磺酸甲酯诱导的耐羟基脲DNA合成。碱性蔗糖梯度沉降分析显示，在紫外线照射的HCMV感染的Vero细胞中，所有大小类别的DNA合成速率均增强。然而，与紫外线或甲磺酸甲酯不同，HCMV感染未在细胞DNA中诱导可修复的损伤，也未显著抑制宿主细胞DNA合成。这些结果表明，HCMV增强了宿主细胞的DNA修复能力，而未在细胞DNA中产生可检测到的损伤，也未抑制DNA合成。当用单纯疱疹病毒胸苷激酶阴性突变体进行测试时，这种修复对于紫外线损伤的单纯疱疹病毒DNA似乎是无误的。
