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Acta Vet Scand. 1981;22(1):32-8. doi: 10.1186/BF03547203.
2
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Isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin.在胰蛋白酶存在的情况下，通过细胞转瓶培养分离细胞病变性猪轮状病毒。
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Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.用于检测儿童肠胃炎病毒的直接电子显微镜检查、免疫电子显微镜检查和轮状病毒酶联免疫吸附测定的比较
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Detection of rotavirus in experimentally infected piglets.
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引用本文的文献

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Epidemiological studies of piglet diarrhoea in intensively managed Danish sow herds. IV. Pathogenicity of porcine rotavirus.丹麦集约化管理母猪群仔猪腹泻的流行病学研究。IV. 猪轮状病毒的致病性
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本文引用的文献

1
Optimized enzyme-linked immunosorbent assay for detection of human and bovine rotavirus in stools: Comparison with electron-microscopy, immunoelectro-osmophoresis, and fluorescent antibody techniques.用于检测粪便中人和牛轮状病毒的优化酶联免疫吸附测定：与电子显微镜、免疫电渗电泳和荧光抗体技术的比较
J Med Virol. 1981;7(1):29-40. doi: 10.1002/jmv.1890070104.
2
Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.对流免疫电渗泳法检测婴幼儿胃肠炎病毒（轮状病毒组）抗原和抗体
J Clin Pathol. 1976 Mar;29(3):191-7. doi: 10.1136/jcp.29.3.191.
3
Letter: Demonstration of reovirus-like particles in intestinal contents of piglets with diarrhoea.信件：在腹泻仔猪肠道内容物中发现呼肠孤病毒样颗粒
Aust Vet J. 1975 Nov;51(11):536. doi: 10.1111/j.1751-0813.1975.tb06917.x.
4
Reovirus-like agent associated with fatal diarrhea in neonatal pigs.与新生仔猪致命性腹泻相关的呼肠孤病毒样病原体。
Infect Immun. 1976 Sep;14(3):816-25. doi: 10.1128/iai.14.3.816-825.1976.
5
Rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis.轮状病毒感染的快速诊断：电子显微镜检查与免疫电渗电泳法在检测人类婴幼儿肠胃炎中轮状病毒的比较
J Gen Virol. 1977 May;35(2):203-18. doi: 10.1099/0022-1317-35-2-203.
6
Experimental infection of pigs with Belgian isolates of the porcine rotavirus.用猪轮状病毒比利时分离株对猪进行实验性感染。
Zentralbl Veterinarmed B. 1979 Sep;26(7):517-26. doi: 10.1111/j.1439-0450.1979.tb00845.x.
7
[Rotavirus detection in the feces: experiences with the enzyme-linked immunosorbent assay (ELISA)].粪便中轮状病毒的检测：酶联免疫吸附测定（ELISA）的经验
Zentralbl Veterinarmed B. 1979 Dec;26(10):835-42.
8
Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.用于检测犊牛粪便中轮状病毒抗原的五种诊断方法的比较
Med Microbiol Immunol. 1978 Nov 17;166(1-4):157-63. doi: 10.1007/BF02121145.
9
Diarrhoea in piglets inoculated with rotavirus.接种轮状病毒的仔猪腹泻
Aust Vet J. 1978 Apr;54(4):188-92. doi: 10.1111/j.1751-0813.1978.tb02447.x.
10
Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs.实验接种无菌猪的猪轮状病毒感染的发病机制
Am J Vet Res. 1978 Feb;39(2):213-20.

通过电子显微镜、酶联免疫吸附测定和补体结合免疫电泳技术检测猪轮状病毒。

Detection of porcine rotavirus by EM, ELISA and CIET.

作者信息

Askaa J, Bloch B

出版信息

Acta Vet Scand. 1981;22(1):32-8. doi: 10.1186/BF03547203.

DOI:10.1186/BF03547203
PMID:6266239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8300443/
Abstract

Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.

摘要

采用电子显微镜（EM）、酶联免疫吸附测定（ELISA）和交叉免疫电泳试验（CIET）三种技术，对出现严重新生仔猪腹泻、3周龄腹泻或早期断奶腹泻问题的猪群粪便样本进行了猪轮状病毒检测。约三分之一的粪便样本检测出轮状病毒感染，涉及超过半数的受检猪群。EM和ELISA检测出几乎所有阳性样本，而此处使用的CIET仅检测出其中一半。