通过电子显微镜、酶联免疫吸附测定和补体结合免疫电泳技术检测猪轮状病毒。
Detection of porcine rotavirus by EM, ELISA and CIET.
作者信息
Askaa J, Bloch B
出版信息
Acta Vet Scand. 1981;22(1):32-8. doi: 10.1186/BF03547203.
Abstract
Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.
摘要
采用电子显微镜（EM）、酶联免疫吸附测定（ELISA）和交叉免疫电泳试验（CIET）三种技术，对出现严重新生仔猪腹泻、3周龄腹泻或早期断奶腹泻问题的猪群粪便样本进行了猪轮状病毒检测。约三分之一的粪便样本检测出轮状病毒感染，涉及超过半数的受检猪群。EM和ELISA检测出几乎所有阳性样本，而此处使用的CIET仅检测出其中一半。
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2
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3
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