用于检测犊牛粪便中轮状病毒抗原的五种诊断方法的比较
Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.
作者信息
Ellens D J, de Leeuw P W, Straver P J, van Balken J A
出版信息
Med Microbiol Immunol. 1978 Nov 17;166(1-4):157-63. doi: 10.1007/BF02121145.
Abstract
Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing.
摘要
对免疫电渗电泳、补体结合试验、接种细胞培养物的免疫荧光法、电子显微镜检查以及酶联免疫吸附测定法进行了比较，以检测犊牛粪便样本中的轮状病毒。通过电子显微镜检查，在98份粪便样本中的39份中检测到了轮状病毒颗粒。免疫荧光法、补体结合试验和免疫电渗电泳分别在39份、42份和30份样本中检测到了轮状病毒抗原。酶联免疫吸附测定法在49份粪便样本中显示出轮状病毒抗原。该测定法不仅灵敏，而且操作简单，适用于大规模检测。
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