Kruiswijk T, Planta R J, Krop J M
Biochim Biophys Acta. 1978 Feb 16;517(2):378-89. doi: 10.1016/0005-2787(78)90204-6.
The course of the assembly of the various ribosomal proteins of yeast into ribosomal particles has been studied by following the incorporation of radioactive individual protein species in cytoplasmic ribosomal particles after pulse-labelling of yeast protoplasts with tritiated amino acids. The pool of ribosomal proteins is small relative to the rate of ribosomal protein synthesis, and, therefore, does not affect essentially the appearance of labelled ribosomal proteins on the ribosomal particles. From the labelling kinetics of individual protein species it can be concluded that a number of ribosomal proteins of the 60 S subunit (L6, L7, L8, L9, L11, L15, L16, L23, L24, L30, L32, L36, L40, L41, L42, L44 and L45) associate with the ribonucleoprotein particles at a relatively late stage of the ribosomal maturation process. The same was found to be true for a number of proteins of the 40 S ribosomal subunit (S10, S27, S31, S32, S33 and S34). Several members (L7, L9, L24 and L30) of the late associating group of 60-S subunit proteins were found to be absent from a nuclear 66 S precursor ribosomal fraction. These results indicate that incorporation of these proteins into the ribosomal particles takes place in the cytoplasm at a late stage of the ribosomal maturation process.
通过用氚标记的氨基酸对酵母原生质体进行脉冲标记后，追踪放射性单个蛋白质种类在细胞质核糖体颗粒中的掺入情况，研究了酵母各种核糖体蛋白组装成核糖体颗粒的过程。核糖体蛋白库相对于核糖体蛋白合成速率较小，因此基本上不影响核糖体颗粒上标记核糖体蛋白的出现。从单个蛋白质种类的标记动力学可以得出结论，60S亚基的一些核糖体蛋白（L6、L7、L8、L9、L11、L15、L16、L23、L24、L30、L32、L36、L40、L41、L42、L44和L45）在核糖体成熟过程的相对晚期与核糖核蛋白颗粒结合。40S核糖体亚基的一些蛋白质（S10、S27、S31、S32、S33和S34）也被发现是这样。发现60S亚基蛋白质的晚期结合组中的几个成员（L7、L9、L24和L30）在核66S前体核糖体组分中不存在。这些结果表明，这些蛋白质在核糖体成熟过程的晚期在细胞质中掺入核糖体颗粒。
